EMAIL THIS PAGE TO A FRIEND

Toxicology letters

Inhibition of cathepsin S induces autophagy and apoptosis in human glioblastoma cell lines through ROS-mediated PI3K/AKT/mTOR/p70S6K and JNK signaling pathways.


PMID 24875536

Abstract

Cathepsin S is a lysosomal cysteine protease that is overexpressed in various cancer models and plays important role in tumorigenesis, however the mechanisms are unclear. In the present study, we found that inhibition of cathepsin S induced autophagy and mitochondrial apoptosis in human glioblastoma cells. Blockade of autophagy by either a chemical inhibitor or RNA interference attenuated cathespin S inhibition-induced apoptosis. Furthermore, autophagy and apoptosis induction was dependent on the suppression of phosphatidylinositide 3-kinases/protein kinase B/mammalian target of rapamycin/p70S6 kinase (PI3K/AKT/mTOR/p70S6K) signaling pathway and activation of c-Jun N-terminal kinase (JNK) signaling pathway. In addition, reactive oxygen species (ROS) served as an upstream of PI3K/AKT/mTOR/p70S6K and JNK signaling pathways. In conclusion, the current study revealed that cathepsin S played an important role in the regulation of autophagy and apoptosis in human glioblastoma cells.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

1009005
Acetylcysteine, United States Pharmacopeia (USP) Reference Standard
C5H9NO3S
A0150000
Acetylcysteine, European Pharmacopoeia (EP) Reference Standard
C5H9NO3S
SAB1305642
ANTI-APG5 antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
PRS4421
Anti-ATG12 (ab1) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
PRS4423
Anti-ATG12 (ab2) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB1406784
Anti-ATG12 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
A8731
Anti-Atg12 antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
SAB2107360
Anti-ATG12 antibody produced in rabbit, affinity isolated antibody
A3231
Anti-ATG3 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB3500500
Anti-ATG3 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
A0856
Anti-ATG5 (N-terminal) antibody produced in rabbit, affinity isolated antibody, PBS solution
GW22210F
Anti-ATG5 antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
SAB3500448
Anti-ATG5 antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
HPA042973
Anti-ATG5 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1407006
Anti-ATG7 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
A2856
Anti-ATG7 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
HPA007639
Anti-ATG7 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1410101
Anti-ATG7 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
HPA001302
Anti-CASP8 antibody produced in rabbit, Ab3, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA005688
Anti-CASP8 antibody produced in rabbit, Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
GW21154 Anti-Caspase 8 antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
SAB3500404
Anti-Caspase-8 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB1408920
Anti-CTSS antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA002988
Anti-CTSS antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1401098
Anti-CTSS antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
SAB2701995
Anti-CTSS antibody produced in rabbit
SAB1410987
Anti-CYCS antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
C5723 Anti-Cytochrome c antibody produced in sheep, whole antiserum
C9616
Anti-Cytochrome c antibody produced in sheep, ~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution
SAB2701263
Anti-DCTN4 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution