EMAIL THIS PAGE TO A FRIEND

Nature communications

Snf2h-mediated chromatin organization and histone H1 dynamics govern cerebellar morphogenesis and neural maturation.


PMID 24946904

Abstract

Chromatin compaction mediates progenitor to post-mitotic cell transitions and modulates gene expression programs, yet the mechanisms are poorly defined. Snf2h and Snf2l are ATP-dependent chromatin remodelling proteins that assemble, reposition and space nucleosomes, and are robustly expressed in the brain. Here we show that mice conditionally inactivated for Snf2h in neural progenitors have reduced levels of histone H1 and H2A variants that compromise chromatin fluidity and transcriptional programs within the developing cerebellum. Disorganized chromatin limits Purkinje and granule neuron progenitor expansion, resulting in abnormal post-natal foliation, while deregulated transcriptional programs contribute to altered neural maturation, motor dysfunction and death. However, mice survive to young adulthood, in part from Snf2l compensation that restores Engrailed-1 expression. Similarly, Purkinje-specific Snf2h ablation affects chromatin ultrastructure and dendritic arborization, but alters cognitive skills rather than motor control. Our studies reveal that Snf2h controls chromatin organization and histone H1 dynamics for the establishment of gene expression programs underlying cerebellar morphogenesis and neural maturation.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

07-274
Anti-dimethyl-Histone H3 (Lys36) Antibody, 200 µL, Use Anti-dimethyl-Histone H3 (Lys36) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB to detect dimethyl-Histone H3 (Lys36) also known as H3K36me2, Histone H3 (di methyl K36).
MAB377
Anti-NeuN Antibody, clone A60, 500 µg, Anti-NeuN Antibody, clone A60 detects level of NeuN and has been published and validated for use in FC, IC, IF, IH, IH(P), IP and WB.
06-570
Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker, 200 µg, Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker is a Rabbit Polyclonal Antibody for detection of Histone H3 phosphorylated at serine 10.This highly published Ab, also known as Anti-H3S10p, has been validated in ICC, IP & WB.
HPA023099
Anti-CALB1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1408554
Anti-CALB1 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
C2724
Anti-Calbindin-D-28K (EG-20) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB1409834
Anti-CCDC6 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA019049
Anti-CCDC6 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab1
HPA019051
Anti-CCDC6 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab2
SAB2107667
Anti-CCDC6 antibody produced in rabbit, affinity isolated antibody
SAB1411455
Anti-CCDC6 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
SAB1410334
Anti-EMP3 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
SAB2104364
Anti-EN1 antibody produced in rabbit, affinity isolated antibody
SAB2107843
Anti-EN1 antibody produced in rabbit, affinity isolated antibody
SAB2700999
Anti-H2AFZ antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB2701003
Anti-H2AFZ antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
AV46122
Anti-HIST1H1C antibody produced in rabbit, affinity isolated antibody
SAB2701559
Anti-HIST1H1C antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
AV51481
Anti-HIST2H2AA3 antibody produced in rabbit, IgG fraction of antiserum
SAB1305308
ANTI-HIST2H2AC (CENTER) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
AV51480
Anti-HIST2H2AC antibody produced in rabbit, IgG fraction of antiserum, lyophilized powder
SAB4300619
Anti-HIST2H2BE antibody produced in rabbit, affinity isolated antibody
SAB2107598
Anti-HIST2H4 antibody produced in rabbit, affinity isolated antibody
SAB4500408
Anti-Histone H1 (Acetyl-Lys25) antibody produced in rabbit, affinity isolated antibody
SAB4500348
Anti-Histone H3 (Acetyl-Lys14) antibody produced in rabbit, affinity isolated antibody
SAB4500349
Anti-Histone H3 (Acetyl-Lys18) antibody produced in rabbit, affinity isolated antibody
SAB4500350
Anti-Histone H3 (Acetyl-Lys23) antibody produced in rabbit, affinity isolated antibody
SAB4500351
Anti-Histone H3 (Acetyl-Lys27) antibody produced in rabbit, affinity isolated antibody
SAB4500347
Anti-Histone H3 (Acetyl-Lys9) antibody produced in rabbit, affinity isolated antibody
H9289
Anti-Histone H3 (N-terminal) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution