EMAIL THIS PAGE TO A FRIEND

Analytica chimica acta

Liquid chromatography with diode array detection and multivariate curve resolution for the selective and sensitive quantification of estrogens in natural waters.


PMID 24952625

Abstract

Following the green analytical chemistry principles, an efficient strategy involving second-order data provided by liquid chromatography (LC) with diode array detection (DAD) was applied for the simultaneous determination of estriol, 17β-estradiol, 17α-ethinylestradiol and estrone in natural water samples. After a simple pre-concentration step, LC-DAD matrix data were rapidly obtained (in less than 5 min) with a chromatographic system operating isocratically. Applying a second-order calibration algorithm based on multivariate curve resolution with alternating least-squares (MCR-ALS), successful resolution was achieved in the presence of sample constituents that strongly coelute with the analytes. The flexibility of this multivariate model allowed the quantification of the four estrogens in tap, mineral, underground and river water samples. Limits of detection in the range between 3 and 13 ng L(-1), and relative prediction errors from 2 to 11% were achieved.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

284785
(S)-(+)-6-Methoxy-α-methyl-2-naphthaleneacetic acid, 98%
C14H14O3
N6127
19-Norethindrone acetate
C22H28O3
46527
19-Norethindrone acetate, VETRANAL, analytical standard
C22H28O3
D0940000
Diazepam, European Pharmacopoeia (EP) Reference Standard
C16H13ClN2O
D9900
Diazepam solution, 1.0 mg/mL in methanol, analytical standard, for drug analysis
C16H13ClN2O
SML0147
Drospirenone, ≥98% (HPLC)
C24H30O3
Y0001105
Drospirenone, European Pharmacopoeia (EP) Reference Standard
C24H30O3
E1253
Estriol, ≥97%
C18H24O3
E1149
Estriol, meets USP testing specifications
C18H24O3
46565
Estriol, VETRANAL, analytical standard
C18H24O3
285803
Estriol, 98%
C18H24O3
E1680000
Estriol, European Pharmacopoeia (EP) Reference Standard
C18H24O3
E9750
Estrone, ≥99%
C18H22O2
E1274
Estrone, meets USP testing specifications
C18H22O2
46573
Estrone, VETRANAL, analytical standard
C18H22O2
1255001
Estrone, United States Pharmacopeia (USP) Reference Standard
C18H22O2
E1700000
Estrone, European Pharmacopoeia (EP) Reference Standard
C18H22O2
PHR1535
Estrone, Pharmaceutical Secondary Standard; Certified Reference Material
C18H22O2
N8280
Naproxen, meets USP testing specifications
C14H14O3
46482
Naproxen, VETRANAL, analytical standard
C14H14O3
PHR1040
Naproxen, Pharmaceutical Secondary Standard; Certified Reference Material
C14H14O3
N0250000
Naproxen, European Pharmacopoeia (EP) Reference Standard
C14H14O3
1457301
Naproxen, United States Pharmacopeia (USP) Reference Standard
C14H14O3
N1225000
Norethisterone acetate, European Pharmacopoeia (EP) Reference Standard
C22H28O3
Y0001532
Norethisterone acetate for system suitability, European Pharmacopoeia (EP) Reference Standard
C22H28O3