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Anaerobe

Inxa0vitro activity of MCB3681 against Clostridium difficile strains.


PMID 25016084

Abstract

One hundred fourteen Clostridium difficile strains were collected from 67 patients and analyzed for the presence of C.xa0difficile toxin B by the cell cytotoxoicity neutralization assay, genes for toxin A, toxin B, binary toxin and TcdC deletion by PCR. All strains were also PCR-ribotyped. The MICs of the isolates were determined against MCB3681 and nine other antimicrobial agents by the agar dilution method. All isolates were positive for toxin B as well as for toxin A and B genes. In addition, 13 isolates were positive for the binary toxin genes. Thirty-two different ribotypes were identified. No strain of ribotype 027 was found. All 114 isolates were sensitive to MCB3681 (0.008-0.5xa0mg/l), cadazolid (0.064-0.5xa0mg/l), fidaxomicin (0.008-0.125xa0mg/l), metronidazole (0.125-2xa0mg/l), vancomycin (0.125-1xa0mg/l) and tigecycline (0.032-0.25xa0mg/l). Three isolates were resistant to linezolid (8xa0mg/l), 12 isolates were resistant to moxifloxacin (8-32xa0mg/l), 87 isolates were resistant to clindamycin (8-256xa0mg/l) and 107 isolates were resistant to ciprofloxacin (8-256xa0mg/l). No association between toxins A, B and binary toxin, ribotypes and the sensitivity to MCB3681 could be found. MCB3681 has a potent inxa0vitro activity against C.xa0difficile.