EMAIL THIS PAGE TO A FRIEND

International journal of oncology

Genetic screen identifies suppressor of morphogenesis in genitalia-1 (SMG-1) as a modulator of sorafenib resistance in hepatocellular carcinoma cell lines.


PMID 25017961

Abstract

Hepatocellular carcinoma (HCC) is an aggressive malignancy with a poor prognosis and a very complex dysregulated molecular etiology. Furthermore, conventional therapy thus far has had only limited success. A recently developed oral multikinase inhibitor, sorafenib, has been used to improve survival in HCC patients, however, follow‑up studies have revealed a high rate of cancer recurrence. Therefore, identification of genes involved in sorafenib resistance is urgently required. RNA interference (RNAi) is a powerful tool for performing loss-of-function genetic screens and can facilitate the identification of components of the cellular signaling pathway. This study describes the results of an unbiased genomic screening using RNAi in an HCC cell line to elucidate genes related to sorafenib non-responsiveness or resistance. A genome-wide inxa0vitro RNA interference screen revealed the role of suppressor of morphogenesis in genitalia-1 (SMG-1) as a determinant of sorafenib resistance. The inhibition of SMG-1 reduced sorafenib sensitivity in the studied HCC cell lines. An immunohistochemical comparison of cancerous and non‑cancerous regions showed strong staining in the non‑neoplastic hepatocyte regions of HCC. SMG-1 may warrant investigation as an agent to reverse sorafenib resistance.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB1407170
Anti-SAMD4A antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA043061
Anti-SAMD4A antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1412284
ANTI-SMG1 antibody produced in mouse, clone 1C12, purified immunoglobulin, buffered aqueous solution
SAB1412637
ANTI-SMG1 antibody produced in mouse, clone 2B10, purified immunoglobulin, buffered aqueous solution
SAB1412638
ANTI-SMG1 antibody produced in mouse, clone 4E4, purified immunoglobulin, buffered aqueous solution
HPA006870
Anti-SMG1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1401580
Monoclonal Anti-SAMD4A antibody produced in mouse, clone 1A4, purified immunoglobulin, buffered aqueous solution
WH0023049M1
Monoclonal Anti-SMG1 antibody produced in mouse, clone 1G5, purified immunoglobulin, buffered aqueous solution
SAB1404950
Monoclonal Anti-SMG1 antibody produced in mouse, clone 1A8, purified immunoglobulin, buffered aqueous solution
WH0006637M1
Monoclonal Anti-SNRPG antibody produced in mouse, clone 2H8-1C12, purified immunoglobulin, buffered aqueous solution
SAB1401368
Monoclonal Anti-SNRPG antibody produced in mouse, clone 2F1-1F12, purified immunoglobulin, buffered aqueous solution
PLA0194
Rabbit anti-SMG1 Antibody, Affinity Purified, Powered by Bethyl Laboratories, Inc.