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FEMS microbiology ecology

Characterization of Pythium oligandrum populations that colonize the rhizosphere of vines from the Bordeaux region.


PMID 25041717

Abstract

This study focused on one oomycete, Pythium oligandrum, well-known for its plant protection abilities, which thrives in microbial environment where bacteria and fungal communities are also present. The genetic structures and dynamics of fungal and bacterial communities were studied in three Bordeaux subregions with various types of soil, using single-strand conformation polymorphism. The structure of the fungal communities colonizing the rhizosphere of vines planted in sandy-stony soils was markedly different from that those planted in silty and sandy soils; such differences were not observed for bacteria. In our 2-year experiment, the roots of all the vine samples were also colonized by echinulated oospore Pythium species, with P.xa0oligandrum predominating. Cytochrome oxidase I and tubulin gene sequencings showed that P.xa0oligandrum strains clustered into three groups. Based on elicitin-like genes coding for proteins able to induce plant resistance, six populations were identified. However, none of these groups was assigned to a particular subregion of Bordeaux vineyards, suggesting that these factors do not shape the genetic structure of P.xa0oligandrum populations. Results showed that different types of rootstock and weeding management both influence root colonization by P.xa0oligandrum. These results should prove particularly useful in improving the management of potentially plant-protective microorganisms.