EMAIL THIS PAGE TO A FRIEND

BMC genomics

Functional transcriptome analysis of the postnatal brain of the Ts1Cje mouse model for Down syndrome reveals global disruption of interferon-related molecular networks.


PMID 25052193

Abstract

The Ts1Cje mouse model of Down syndrome (DS) has partial triplication of mouse chromosome 16 (MMU16), which is partially homologous to human chromosome 21. These mice develop various neuropathological features identified in DS individuals. We analysed the effect of partial triplication of the MMU16 segment on global gene expression in the cerebral cortex, cerebellum and hippocampus of Ts1Cje mice at 4 time-points: postnatal day (P)1, P15, P30 and P84. Gene expression profiling identified a total of 317 differentially expressed genes (DEGs), selected from various spatiotemporal comparisons, between Ts1Cje and disomic mice. A total of 201 DEGs were identified from the cerebellum, 129 from the hippocampus and 40 from the cerebral cortex. Of these, only 18 DEGs were identified as common to all three brain regions and 15 were located in the triplicated segment. We validated 8 selected DEGs from the cerebral cortex (Brwd1, Donson, Erdr1, Ifnar1, Itgb8, Itsn1, Mrps6 and Tmem50b), 18 DEGs from the cerebellum (Atp5o, Brwd1, Donson, Dopey2, Erdr1, Hmgn1, Ifnar1, Ifnar2, Ifngr2, Itgb8, Itsn1, Mrps6, Paxbp1, Son, Stat1, Tbata, Tmem50b and Wrb) and 11 DEGs from the hippocampus (Atp5o, Brwd1, Cbr1, Donson, Erdr1, Itgb8, Itsn1, Morc3, Son, Tmem50b and Wrb). Functional clustering analysis of the 317 DEGs identified interferon-related signal transduction as the most significantly dysregulated pathway in Ts1Cje postnatal brain development. RT-qPCR and western blotting analysis showed both Ifnar1 and Stat1 were over-expressed in P84 Ts1Cje cerebral cortex and cerebellum as compared to wild type littermates. These findings suggest over-expression of interferon receptor may lead to over-stimulation of Jak-Stat signaling pathway which may contribute to the neuropathology in Ts1Cje or DS brain. The role of interferon mediated activation or inhibition of signal transduction including Jak-Stat signaling pathway has been well characterized in various biological processes and disease models including DS but information pertaining to the role of this pathway in the development and function of the Ts1Cje or DS brain remains scarce and warrants further investigation.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB1406003
Anti-IFNAR1 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA018015
Anti-IFNAR1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1406004
Anti-IFNAR2 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
SAB1401203
Anti-IFNAR2 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
SAB2700886
Anti-IFNAR2 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
I4902 Anti-Interferon-α/β Receptor 1 antibody produced in goat, affinity isolated antibody, lyophilized powder
SAB1105169 Anti-STAT1 (671-685) antibody produced in rabbit, IgG fraction of antiserum
SAB4300326
Anti-STAT1 (Ab-701) antibody produced in rabbit, affinity isolated antibody
SAB4300441
Anti-STAT1 (Ab-727) antibody produced in rabbit, affinity isolated antibody
GW22758 Anti-STAT1 (ab1) antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
AV33373
Anti-STAT1 (AB1) antibody produced in rabbit, affinity isolated antibody
GW22780 Anti-STAT1 (ab2) antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
AV33374
Anti-STAT1 (AB2) antibody produced in rabbit, affinity isolated antibody
AV38933
Anti-STAT1 (AB3) antibody produced in rabbit, affinity isolated antibody
AV38934
Anti-STAT1 (AB4) antibody produced in rabbit, affinity isolated antibody
SAB1406486
Anti-STAT1 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA000931
Anti-STAT1 antibody produced in rabbit, Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AV36979
Anti-STAT1 antibody produced in rabbit, IgG fraction of antiserum
S5683 Anti-STAT1 antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous glycerol solution
HPA000982
Anti-STAT1 antibody produced in rabbit, Ab2, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
WH0003454M1
Monoclonal Anti-IFNAR1 antibody produced in mouse, clone 1H2, purified immunoglobulin, buffered aqueous solution
WH0006772M1
Monoclonal Anti-STAT1 antibody produced in mouse, clone 1A8, purified immunoglobulin, buffered aqueous solution