EMAIL THIS PAGE TO A FRIEND

Basic research in cardiology

MicroRNA-144 is a circulating effector of remote ischemic preconditioning.


PMID 25060662

Abstract

Remote ischemic preconditioning (rIPC) induced by cycles of transient limb ischemia and reperfusion is a powerful cardioprotective strategy with additional pleiotropic effects. However, our understanding of its underlying mediators and mechanisms remains incomplete. We examined the role of miR-144 in the cardioprotection induced by rIPC. Microarray studies first established that rIPC increases, and IR injury decreases miR-144 levels in mouse myocardium, the latter being rescued by both rIPC and intravenous administration of miR-144. Going along with this systemic treatment with miR-144 increased P-Akt, P-GSK3β and P-p44/42 MAPK, decreased p-mTOR level and induced autophagy signaling, and induced early and delayed cardioprotection with improved functional recovery and reduction in infarct size similar to that achieved by rIPC. Conversely, systemic administration of a specific antisense oligonucleotide reduced myocardial levels of miR-144 and abrogated cardioprotection by rIPC. We then showed that rIPC increases plasma miR-144 levels in mice and humans, but there was no change in plasma microparticle (50-400 nM) numbers or their miR-144 content. However, there was an almost fourfold increase in miR-144 precursor in the exosome pellet, and a significant increase in miR-144 levels in exosome-poor serum which, in turn, was associated with increased levels of the miR carriage protein Argonaute-2. Systemic release of microRNA 144 plays a pivotal role in the cardioprotection induced by rIPC. Future studies should assess the potential for plasma miR-144 as a biomarker of the effectiveness of rIPC induced by limb ischemia, and whether miR-144 itself may represent a novel therapy to reduce clinical ischemia-reperfusion injury.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

AV36578
Anti-ANXA3 (AB1) antibody produced in rabbit, IgG fraction of antiserum
AV36579
Anti-ANXA3 (AB2) antibody produced in rabbit, IgG fraction of antiserum
SAB1405458
Anti-ANXA3 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA013398
Anti-ANXA3 antibody produced in rabbit, Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA013431
Anti-ANXA3 antibody produced in rabbit, Ab2, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
B3773 Anti-Human IgG (Fc specific)−Biotin antibody, Mouse monoclonal, clone HP-6017, purified from hybridoma cell culture
SAB1305638 ANTI-LC3 (APG8A) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
SAB1408113
Anti-MAP1LC3A antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
SAB2101425
Anti-MAP1LC3A antibody produced in rabbit, affinity isolated antibody
SAB4300571
Anti-MAP1LC3A antibody produced in rabbit, affinity isolated antibody
SAB1411514
Anti-MAP1LC3A antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
WH0000306M12
Monoclonal Anti-ANXA3 antibody produced in mouse, clone 4F1, purified immunoglobulin, buffered aqueous solution
SAB1403553
Monoclonal Anti-ANXA3 antibody produced in mouse, clone 1E2, purified immunoglobulin, buffered aqueous solution
SAB4100143
Monoclonal Anti-ANXA3 antibody produced in mouse, clone ANXA3-A107, culture supernatant
G2904 Monoclonal Anti-Goat/Sheep IgG antibody produced in mouse, clone GT-34, ascites fluid
A8062 Monoclonal Anti-Goat/Sheep IgG–Alkaline Phosphatase antibody produced in mouse, clone GT-34, purified immunoglobulin, buffered aqueous glycerol solution
B3148 Monoclonal Anti-Goat/Sheep IgG–Biotin antibody produced in mouse, clone GT-34, purified immunoglobulin, buffered aqueous solution
F4891 Monoclonal Anti-Goat/Sheep IgG−FITC antibody produced in mouse, clone GT-34, purified immunoglobulin, buffered aqueous solution
A9452 Monoclonal Anti-Goat/Sheep IgG–Peroxidase antibody produced in mouse, clone GT-34, purified immunoglobulin, lyophilized powder
I6135 Monoclonal Anti-Human IgG (Fab specific) antibody produced in mouse, clone GG-6, ascites fluid
I6260 Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse, clone GG-7, ascites fluid
I6760 Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse, clone HP-6017, ascites fluid
F5016 Monoclonal Anti-Human IgG (Fc specific)−FITC antibody produced in mouse, clone HP-6017, purified immunoglobulin, buffered aqueous solution
I5885 Monoclonal Anti-Human IgG (γ-chain specific) antibody produced in mouse, clone GG-5, ascites fluid
I9513 Monoclonal Anti-Human IgG2 (Fc specific) antibody produced in mouse, clone HP-6002, ascites fluid
I9888 Monoclonal Anti-Human IgG4 (pFc′ specific) antibody produced in mouse, clone HP-6023, ascites fluid
WH0084557M7
Monoclonal Anti-MAP1LC3A antibody produced in mouse, clone 1D5, purified immunoglobulin, buffered aqueous solution
R0761 Monoclonal Anti-Rat IgG2a antibody produced in mouse, clone R2A-2, ascites fluid