EMAIL THIS PAGE TO A FRIEND

Nucleic acids research

A novel antisense long noncoding RNA within the IGF1R gene locus is imprinted in hematopoietic malignancies.


PMID 25092925

Abstract

Dysregulation of the insulin-like growth factor type I receptor (IGF1R) has been implicated in the progression and therapeutic resistance of malignancies. In acute myeloid leukemia (AML) cells, IGF1R is one of the most abundantly phosphorylated receptor tyrosine kinases, promoting cell growth through the PI3K/Akt signaling pathway. However, little is known regarding the molecular mechanisms underlying IGF1R gene dysregulation in cancer. We discovered a novel intragenic long noncoding RNA (lncRNA) within the IGF1R locus, named IRAIN, which is transcribed in an antisense direction from an intronic promoter. The IRAIN lncRNA was expressed exclusively from the paternal allele, with the maternal counterpart being silenced. Using both reverse transcription-associated trap and chromatin conformation capture assays, we demonstrate that this lncRNA interacts with chromatin DNA and is involved in the formation of an intrachromosomal enhancer/promoter loop. Knockdown of IRAIN lncRNA with shRNA abolishes this intrachromosomal interaction. In addition, IRAIN was downregulated both in leukemia cell lines and in blood obtained from high-risk AML patients. These data identify IRAIN as a new imprinted lncRNA that is involved in long-range DNA interactions.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB1408196
Anti-ATPIF1 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
SAB2100188
Anti-ATPIF1 antibody produced in rabbit, affinity isolated antibody
HPA027999
Anti-ATPIF1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA000426
Anti-IKBKG antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AV30005
Anti-IKBKG antibody produced in rabbit, IgG fraction of antiserum
SAB2103956
Anti-IKBKG, (N-terminal) antibody produced in rabbit, affinity isolated antibody
SAB4503404
Anti-IKK-γ antibody produced in rabbit, affinity isolated antibody
GW21172 Anti-NEMO antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
P7622 Anti-Prostacyclin Receptor antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB4502486
Anti-Prostacyclin Receptor antibody produced in rabbit, affinity isolated antibody
SAB2500916
Anti-SDHB antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB2102103
Anti-SDHB antibody produced in rabbit, affinity isolated antibody
HPA002868
Anti-SDHB antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2106796
Anti-SDHB antibody produced in rabbit, affinity isolated antibody
SAB1411242
Anti-SDHB antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
WH0093974M1
Monoclonal Anti-ATPIF1 antibody produced in mouse, clone 3E2, purified immunoglobulin, buffered aqueous solution
WH0008517M1
Monoclonal Anti-IKBKG antibody produced in mouse, clone 4G9, purified immunoglobulin, buffered aqueous solution
SAB1404591
Monoclonal Anti-IKBKG, (N-terminal) antibody produced in mouse, clone 1D4, purified immunoglobulin, buffered aqueous solution
WH0005739M1
Monoclonal Anti-PTGIR antibody produced in mouse, clone 4B10, purified immunoglobulin, buffered aqueous solution
AMAB90708
Monoclonal Anti-SDHB antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL0349, purified immunoglobulin, buffered aqueous glycerol solution
AMAB90706
Monoclonal Anti-SDHB antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL0347, purified immunoglobulin, buffered aqueous glycerol solution