EMAIL THIS PAGE TO A FRIEND

Journal of chromatography. A

Evaluation of stationary phases packed with superficially porous particles for the analysis of pharmaceutical compounds using supercritical fluid chromatography.


PMID 25129391

Abstract

Superficially porous particles (SPP), or core shell particles, which consist of a non-porous silica core surrounded by a thin shell of porous silica, have gained popularity as a solid support for chromatography over the last decade. In the present study, five unbonded silica, one diol, and two ethylpyridine (2-ethyl and 4-ethyl) SPP columns were evaluated under SFC conditions using two mixtures, one with 17 drug-like compounds and the other one with 7 drug-like basic compounds. Three of the SPP phases, SunShell™ 2-ethylpyridine (2-EP), Poroshell™ HILIC, and Ascentis(®) Express HILIC, exhibited superior performances relative to the others (reduced theoretical plate height (hmin) values of 1.9-2.5 for neutral compounds). When accounting for both achievable plate count and permeability of the support using kinetic plot evaluation, the Cortecs™ HILIC 1.6μm and Ascentis(®) Express HILIC 2.7μm phases were found to be the best choices among tested SPPs to reach efficiencies up to 30,000 plates in the minimum amount of time. For desired efficiencies ranging from 30,000 to 60,000 plates, the SunShell™ 2-EP 2.6μm column clearly outperformed all other SPPs. With the addition of a mobile phase additive such as 10mM ammonium formate, which was required to elute the basic components with sharp peaks, the Poroshell™ HILIC, SunShell™ Diol and SunShell™ 2-EP phases represent the most orthogonal SPP columns with the highest peak capacities. This study demonstrates the obvious benefits of using columns packed with SPP on current SFC instrumentation.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

284785
(S)-(+)-6-Methoxy-α-methyl-2-naphthaleneacetic acid, 98%
C14H14O3
112429
2-Ethylpyridine, 97%
C7H9N
A7302
4-Acetamidophenol, 98%
C8H9NO2
112437
4-Ethylpyridine, 98%
C7H9N
A7085
Acetaminophen, BioXtra, ≥99.0%
C8H9NO2
A3035
Acetaminophen, analytical standard
C8H9NO2
A5000
Acetaminophen, meets USP testing specifications, 98.0-102.0%, powder
C8H9NO2
1003009
Acetaminophen, United States Pharmacopeia (USP) Reference Standard
C8H9NO2
PHR1005
Acetaminophen, Pharmaceutical Secondary Standard; Certified Reference Material
C8H9NO2
A-064
Acetaminophen solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material
C8H9NO2
A8800
Alprazolam
C17H13ClN4
A0357000
Alprazolam, European Pharmacopoeia (EP) Reference Standard
C17H13ClN4
A-903
Alprazolam solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material
C17H13ClN4
516961
Ammonium formate, ≥99.995% trace metals basis
CH5NO2
09735
Ammonium formate, BioUltra, ≥99.0% (calc. based on dry substance, NT)
CH5NO2
70221
Ammonium formate, for mass spectrometry, ≥99.0%
CH5NO2
78314
Ammonium formate solution, BioUltra, 10 M in H2O
CH5NO2
714690
Ammonium formate solution, 10 mM in H2O, for HPLC
CH5NO2
E1501
Benzocaine
C9H11NO2
06952
Benzocaine, tested according to Ph.Eur.
C9H11NO2
PHR1158
Benzocaine, Pharmaceutical Secondary Standard; Certified Reference Material
C9H11NO2
B0600000
Benzocaine, European Pharmacopoeia (EP) Reference Standard
C9H11NO2
1054000
Benzocaine, United States Pharmacopeia (USP) Reference Standard
C9H11NO2
54680
Butyl 4-hydroxybenzoate, ≥99.0% (GC)
C11H14O3
W220302
Butyl 4-hydroxybenzoate, ≥99%
C11H14O3
B1217000 Butyl parahydroxybenzoate, European Pharmacopoeia (EP) Reference Standard
C11H14O3
1084000 Butylparaben, United States Pharmacopeia (USP) Reference Standard
C11H14O3
PHR1022 Butylparaben, Pharmaceutical Secondary Standard; Certified Reference Material
C11H14O3
93784
Caffeine, PharmaGrade, EP, Manufactured under appropriate GMP controls for pharma or biopharmaceutical production
C8H10N4O2
C0750
Caffeine, powder, ReagentPlus®
C8H10N4O2