EMAIL THIS PAGE TO A FRIEND

The Journal of clinical endocrinology and metabolism

Prolonged pregnancy in women is associated with attenuated myometrial expression of progesterone receptor co-regulator Krüppel-like Factor 9.


PMID 25313913

Abstract

Late-term pregnancy may lead to maternal and neonatal morbidity and mortality. Mice null for the progesterone receptor co-regulator Krüppel-like Factor 9 (KLF9) exhibit delayed parturition and increased incidence of neonatal deaths. Our aim is to evaluate the contribution of myometrial KLF9 to human parturition. Myometrial biopsies were obtained from women with term (>37 to ≤41 wk) and late-term (>41 wk) pregnancies during cesarean delivery and assessed for gene and protein expression. Human myometrial cells transfected with nontargeting or KLF9 small interfering RNAs (siRNA) were treated with the progesterone antagonist RU486 and analyzed for pro-inflammatory chemokine/cytokine gene expression. The study took place in a University-affiliated tertiary care hospital and University research laboratory. Term patients (n = 8) were in spontaneous active labor whereas late-term patients (n = 5) were either in or were induced to active labor, prior to elective cesarean delivery. Steroid hormone receptor, contractility, and inflammation-associated gene expression in myometrial biopsies and in siKLF9-transfected, RU486-treated human myometrial cells was associated with KLF9 expression levels. Myometrium from women with late-term pregnancy showed lower KLF9, total PGR, and PGR-A/PGR-B isoform expression. Transcript levels of select chemokines/cytokines were up- (CSF3, IL1, IL12A, TGFB2) and down- (CCL3, CCL5, CXCL1, CXCL5, IL15) regulated in late-term relative to term myometrium. Knock-down of KLF9 expression in RU486-treated human myometrial cells modified the expression of PGR and labor-associated cytokines, relative to control siRNA-treated cells. Myometrial KLF9 may contribute to the onset of human parturition through its regulation of PGR expression and inflammatory signaling networks.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB4501766
Anti-Lamin A (Cleaved-Asp230) antibody produced in rabbit, affinity isolated antibody
SAB4501767
Anti-Lamin A (Cleaved-Asp230) antibody produced in rabbit, affinity isolated antibody
SAB4501764
Anti-Lamin A antibody produced in rabbit, affinity isolated antibody
SAB1406087
Anti-LMNA antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
SAB4300348
Anti-PGR (Ab-190) antibody produced in rabbit, affinity isolated antibody
HPA004751
Anti-PGR antibody produced in rabbit, Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AV100687
Anti-PGR antibody produced in rabbit, affinity isolated antibody
SAB2101781
Anti-PGR antibody produced in rabbit, affinity isolated antibody
HPA008428
Anti-PGR antibody produced in rabbit, Ab2, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2105280
Anti-PGR antibody produced in rabbit, affinity isolated antibody
HPA017176
Anti-PGR antibody produced in rabbit, Ab3, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
GW22679 Anti-Progesterone Receptor antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
SAB4502184
Anti-Progesterone Receptor antibody produced in rabbit, affinity isolated antibody
SAB4502185
Anti-Progesterone Receptor antibody produced in rabbit, affinity isolated antibody
SAB4502186
Anti-Progesterone Receptor antibody produced in rabbit, affinity isolated antibody
M1629
Medroxyprogesterone 17-acetate, ≥97% (HPLC)
C24H34O4
46412
Medroxyprogesterone 17-acetate, VETRANAL, analytical standard
C24H34O4
1378001
Medroxyprogesterone acetate, United States Pharmacopeia (USP) Reference Standard
C24H34O4
M0250000
Medroxyprogesterone acetate, European Pharmacopoeia (EP) Reference Standard
C24H34O4
M0250010
Medroxyprogesterone acetate, for performance test, European Pharmacopoeia (EP) Reference Standard
C24H34O4
Y0001484
Medroxyprogesterone acetate for peak identification, European Pharmacopoeia (EP) Reference Standard
C24H34O4
Y0000598
Medroxyprogesterone acetate for system suitability, European Pharmacopoeia (EP) Reference Standard
C24H34O4
M8046
Mifepristone, ≥98%
C29H35NO2
WH0005241M1
Monoclonal Anti-PGR antibody produced in mouse, clone 3E11, purified immunoglobulin, buffered aqueous solution
SAB1400206
Monoclonal Anti-PGR antibody produced in mouse, clone 5D10, purified immunoglobulin, buffered aqueous solution
SAB1400207
Monoclonal Anti-PGR antibody produced in mouse, clone 4E9, purified immunoglobulin, buffered aqueous solution
SAB1404196
Monoclonal Anti-PGR, (N-terminal) antibody produced in mouse, clone 2C2, purified immunoglobulin, buffered aqueous solution