EMAIL THIS PAGE TO A FRIEND

PLoS genetics

BMP-FGF signaling axis mediates Wnt-induced epidermal stratification in developing mammalian skin.


PMID 25329657

Abstract

Epidermal stratification of the mammalian skin requires proliferative basal progenitors to generate intermediate cells that separate from the basal layer and are replaced by post-mitotic cells. Although Wnt signaling has been implicated in this developmental process, the mechanism underlying Wnt-mediated regulation of basal progenitors remains elusive. Here we show that Wnt secreted from proliferative basal cells is not required for their differentiation. However, epidermal production of Wnts is essential for the formation of the spinous layer through modulation of a BMP-FGF signaling cascade in the dermis. The spinous layer defects caused by disruption of Wnt secretion can be restored by transgenically expressed Bmp4. Non-cell autonomous BMP4 promotes activation of FGF7 and FGF10 signaling, leading to an increase in proliferative basal cell population. Our findings identify an essential BMP-FGF signaling axis in the dermis that responds to the epidermal Wnts and feedbacks to regulate basal progenitors during epidermal stratification.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB2700758
Anti-FGF10 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB4300561
Anti-SMAD1 (Ab-465) antibody produced in rabbit, affinity isolated antibody
AV38693
Anti-SMAD1 (AB1) antibody produced in rabbit, IgG fraction of antiserum
AV38692
Anti-SMAD1 (AB2) antibody produced in rabbit, IgG fraction of antiserum
SAB4200377
Anti-SMAD1 (Internal region) antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody
SAB1412181
ANTI-SMAD1 antibody produced in mouse, clone 1C7, purified immunoglobulin, buffered aqueous solution
SAB1412594
ANTI-SMAD1 antibody produced in mouse, clone 3G4, purified immunoglobulin, buffered aqueous solution
SAB1412595
ANTI-SMAD1 antibody produced in mouse, clone 4C12, purified immunoglobulin, buffered aqueous solution
SAB1412656
ANTI-SMAD1 antibody produced in mouse, clone 1B8, purified immunoglobulin, buffered aqueous solution
AV32003
Anti-SMAD1 antibody produced in rabbit, affinity isolated antibody
SAB4200378
Anti-SMAD1 antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody
SAB4501802
Anti-SMAD1 antibody produced in rabbit, affinity isolated antibody
SRP6161
FGF-7 human, recombinant, expressed in human cells, ≥95% (SDS-PAGE)
K1757 Keratinocyte Growth Factor human, KGF, recombinant, expressed in E. coli, powder, suitable for cell culture
SRP3100 KGF human, Animal-component free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), cell culture tested
WH0002255M5
Monoclonal Anti-FGF10 antibody produced in mouse, clone 3C7, purified immunoglobulin, buffered aqueous solution
WH0004086M2
Monoclonal Anti-SMAD1 antibody produced in mouse, clone 1D3, purified immunoglobulin, buffered aqueous solution
SAB1404035
Monoclonal Anti-SMAD1 antibody produced in mouse, clone 2E9, purified immunoglobulin, buffered aqueous solution