EMAIL THIS PAGE TO A FRIEND

Diabetes

High levels of pigment epithelium-derived factor in diabetes impair wound healing through suppression of Wnt signaling.


PMID 25368097

Abstract

Diabetic foot ulcer (DFU) caused by impaired wound healing is a common vascular complication of diabetes. The current study revealed that plasma levels of pigment epithelium-derived factor (PEDF) were elevated in type 2 diabetic patients with DFU and in db/db mice. To test whether elevated PEDF levels contribute to skin wound-healing delay in diabetes, endogenous PEDF was neutralized with an anti-PEDF antibody in db/db mice. Our results showed that neutralization of PEDF accelerated wound healing, increased angiogenesis in the wound skin, and improved the functions and numbers of endothelial progenitor cells (EPCs) in the diabetic mice. Further, PEDF-deficient mice showed higher baseline blood flow in the skin, higher density of cutaneous microvessels, increased skin thickness, improved numbers and functions of circulating EPCs, and accelerated wound healing compared with wild-type mice. Overexpression of PEDF suppressed the Wnt signaling pathway in the wound skin. Lithium chloride-induced Wnt signaling activation downstream of the PEDF interaction site attenuated the inhibitory effect of PEDF on EPCs and rescued the wound-healing deficiency in diabetic mice. Taken together, these results suggest that elevated circulating PEDF levels contribute to impaired wound healing in the process of angiogenesis and vasculogenesis through the inhibition of Wnt/β-catenin signaling.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

02685
Lithium atomic spectroscopy standard concentrate 1.00 g Li, for 1 l standard solution, 1.00 g/L, analytical standard
ClLi
746460
Lithium chloride, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99%
ClLi
793620
Lithium chloride, anhydrous, free-flowing, Redi-Dri, ReagentPlus®, 99%
ClLi
L4408
Lithium chloride, BioXtra, ≥99.0% (titration)
ClLi
62476
Lithium chloride, BioUltra, for molecular biology, anhydrous, ≥99.0% (AT)
ClLi
L9650
Lithium chloride, for molecular biology, ≥99%
ClLi
429457
Lithium chloride, anhydrous, beads, −10 mesh, 99.998% trace metals basis
ClLi
449881
Lithium chloride, anhydrous, beads, −10 mesh, ≥99.9% trace metals basis
ClLi
203637
Lithium chloride, powder, ≥99.99% trace metals basis
ClLi
HT-LICLSLUG
Lithium chloride, anhydrous, chunks, 99.99% trace metals basis
ClLi
751898
Lithium chloride, anhydrous, chunks, 99.99% trace metals basis
ClLi
L7026
Lithium chloride solution, 8 M, for molecular biology, ≥99%
ClLi
694576
Lithium chloride solution, 1 M in 1,3-dimethyl-2-imidazolidinone
ClLi
62483
Lithium chloride solution, 1 M in glacial acetic acid
ClLi
62481
Lithium chloride solution, 1 M in ethanol
ClLi
667544
Lithium chloride solution, 0.5 M in anhydrous tetrahydrofuran
ClLi
LCL30
Lithium chloride solution, 2 M in ethanol
ClLi
601489
Lithium-7Li chloride, 99 atom % 7Li, 99% (CP)
Cl7Li