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European journal of drug metabolism and pharmacokinetics

Placental profiling of UGT1A enzyme expression and activity and interactions with preeclampsia at term.


PMID 25465229

Abstract

Placental UDP-glucuronosyltransferase (UGT) enzymes have critical roles in hormone, nutrient, chemical balance and fetal exposure during pregnancy. Placental UGT1A isoforms were profiled and differences between preeclamptic (PE) and non-PE placental UGT expression determined. In third trimester villous placenta, UGT1A1, 1A4, 1A6 and 1A9 were expressed and active in all specimens (nxa0=xa010), but UGT1A3, 1A5, 1A7, 1A8 and 1A10 were absent. The UGT1A activities were comparable to human liver microsomes per milligram, but placental microsome yields were only 2xa0% of liver (1xa0mg/g of tissue vs. 45xa0mg/g of tissue). For successful PCR, placental collection and processing withinxa060xa0min from delivery, including DNAse and ≥300xa0ng of RNA in reverse transcription were essential and snap freezing in liquid nitrogen immediately was the best preservation method. Although UGT1A6 mRNA was lower in PE (Pxa0

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