EMAIL THIS PAGE TO A FRIEND

British journal of cancer

Characterisation of human thyroid epithelial cells immortalised in vitro by simian virus 40 DNA transfection.


PMID 2557880

Abstract

Human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobulin production). These two lines were characterised in detail and found to be growth factor-independent, capable of anchorage-independent growth at low frequency but non-tumorigenic in nude mice. These differentiated, These differentiated, partially transformed cell lines were shown to be suitable for gene transfer at high frequency using simple coprecipitation techniques.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

90011609
Nthy-ori 3-1 Cell Line human