EMAIL THIS PAGE TO A FRIEND

The Journal of infectious diseases

Interleukin 10-Dominant Immune Response and Increased Risk of Cutaneous Leishmaniasis After Natural Exposure to Lutzomyia intermedia Sand Flies.


PMID 25596303

Abstract

Leishmaniasis is caused by parasites transmitted to the vertebrate host by infected sand flies. During transmission, the vertebrate host is also inoculated with sand fly saliva, which exerts powerful immunomodulatory effects on the host's immune response. We conducted a prospective cohort analysis to characterize the human immune response to Lutzomyia intermedia saliva in 264 individuals, from an area for cutaneous leishmaniasis (CL) caused by Leishmania braziliensis. Antibodies were found in 150 individuals (56.8%); immunoglobulin G1 and G4 were the predominant subclasses. Recall responses to salivary gland sonicate showed elevated production of interleukin 10 (IL-10), interleukin 13, interferon γ, CXCL9, and CCL2 compared with controls. CD4(+)CD25(+) T cells, including Foxp3(+) cells, were the main source of IL-10. L. braziliensis replication was increased (P < .05) in macrophages cocultured with saliva-stimulated lymphocytes from exposed individuals and addition of anti-IL-10 reverted this effect. Positive correlation between antibody response to saliva and cellular response to Leishmania was not found. Importantly, individuals seropositive to saliva are 2.1 times more likely to develop CL (relative risk, 2.1; 95% confidence interval, 1.07-4.2; P < .05). Exposure to L. intermedia sand flies skews the human immune response, facilitating L. braziliensis survival in vitro, and increases the risk of developing CL.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

N9389
4-Nitrophenyl phosphate disodium salt hexahydrate, tablet
C6H4NNa2O6P · 6H2O
N2765
4-Nitrophenyl phosphate disodium salt hexahydrate, tablet
C6H4NNa2O6P · 6H2O
N2640
4-Nitrophenyl phosphate disodium salt hexahydrate, tablet
C6H4NNa2O6P · 6H2O
N4645
4-Nitrophenyl phosphate disodium salt hexahydrate, powder, BioReagent, suitable for cell culture, ≥97%
C6H4NNa2O6P · 6H2O
71768
4-Nitrophenyl phosphate disodium salt hexahydrate, for enzyme immunoassay, ≥99.0% (enzymatic)
C6H4NNa2O6P · 6H2O
SAB1409249
Anti-IL10 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
SAB1410712
Anti-IL10 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
I5020 Anti-Interleukin 10 antibody produced in goat, IgG fraction of antiserum, lyophilized powder
GW21031 Anti-Interleukin-10 antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
I5145 Anti-Interleukin-10 antibody produced in goat, IgG fraction of antiserum, lyophilized powder
SAB1306038
ANTI-TGIF (CENTER L223) antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
GW21970F Anti-TGIF antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
WH0003586M3
Monoclonal Anti-IL10 antibody produced in mouse, clone 1C10, purified immunoglobulin, buffered aqueous solution
SAB5300445
Monoclonal Anti-IL10 antibody produced in mouse, clone 3C12C12, ascites fluid
I7392 Monoclonal Anti-Interleukin-10 antibody produced in mouse, clone 23738.11, purified immunoglobulin, lyophilized powder
WH0007050M1
Monoclonal Anti-TGIF antibody produced in mouse, clone 1D12, purified immunoglobulin, buffered aqueous solution
P4744
Phosphatase substrate, powder
C6H4NNa2O6P · 6H2O
S0942
Phosphatase substrate, 5 mg tablets
C6H4NNa2O6P · 6H2O
P5994
Phosphatase substrate, 40 mg tablets
C6H4NNa2O6P · 6H2O
P5744
Phosphatase substrate, 40 mg capsules
C6H4NNa2O6P · 6H2O
P5869
Phosphatase substrate, 100 mg capsules
C6H4NNa2O6P · 6H2O
SRE0026
Phosphatase substrate, Suitable for manufacturing of diagnostic kits and reagents
C6H4NNa2O6P · 6H2O