EMAIL THIS PAGE TO A FRIEND

Molecular and cellular biochemistry

Caveolin-1 mediates gene transfer and cytotoxicity of polyethyleneimine in mammalian cell lines.


PMID 25626893

Abstract

Polyethyleneimine (PEI) is a cost-effective and non-viral vector for gene transfer,xa0but the factors determining gene transfer efficiency and cytotoxicity of PEI in different mammalian cell lines remain largely unknown. In the present study, three different cell lines were chosen for investigation. Using pEGFP DNA and PEI, 21.5, 29.2, and 92.1xa0% of GFP-positive cells were obtained in BMSC, Hela, and 293T, respectively. In luciferase reporter assay, similar results were obtained (for luciferase activity, BMSCxa0