EMAIL THIS PAGE TO A FRIEND

Endocrinology

Novel three dimensional human endocervix cultures respond to 28-day hormone treatment.


PMID 25635622

Abstract

The endocervix has both anatomical and biological functions that participate in the delicate balance between tolerance necessary for conception and protection from pathogens. Our goal was to develop a robust 3-dimensional (3D) endocervix model that was a reliable representation of the in vivo tissues and to identify the physiological responses to changing levels of steroid hormones during a 28-day time period. Human endocervical cells were grown on polystyrene scaffolds, and the morphologic and hormonal responses of cultured cells were assessed in response to fluctuating levels of estradiol (E2) or progesterone (P4). Morphologically, the 3D cultures were composed of a mixed population of cells, including epithelial and stromal cells. Treatment with E2 and P4 (d 28) increased cell growth and proliferation as compared with no treatment control. Cells expressed estrogen receptor and P4 receptor and produced both neutral and acidic mucins, including Mucin 16. In addition, a 45-plex Luminex assay identified numerous factors secreted and regulated by hormones. Specifically, IL-1β and leukemia inhibitory factor significantly decreased in the presence of E2 and P4 as compared with the no hormone control at day 26. Cotreatment with RU486 (mifepristone) attenuated the inhibition of IL-1β and leukemia inhibitory factor secretion. In summary, a robust, novel 3D endocervical culture was developed, and physiologic responses to the menstrual cycle mimic of E2 and P4 levels for a period of 28 days were identified.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB4300348
Anti-PGR (Ab-190) antibody produced in rabbit, affinity isolated antibody
HPA004751
Anti-PGR antibody produced in rabbit, Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AV100687
Anti-PGR antibody produced in rabbit, affinity isolated antibody
SAB2101781
Anti-PGR antibody produced in rabbit, affinity isolated antibody
HPA008428
Anti-PGR antibody produced in rabbit, Ab2, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2105280
Anti-PGR antibody produced in rabbit, affinity isolated antibody
HPA017176
Anti-PGR antibody produced in rabbit, Ab3, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
GW22679 Anti-Progesterone Receptor antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
SAB4502184
Anti-Progesterone Receptor antibody produced in rabbit, affinity isolated antibody
SAB4502185
Anti-Progesterone Receptor antibody produced in rabbit, affinity isolated antibody
SAB4502186
Anti-Progesterone Receptor antibody produced in rabbit, affinity isolated antibody
HPA053794
Anti-TMEM37 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
WH0005241M1
Monoclonal Anti-PGR antibody produced in mouse, clone 3E11, purified immunoglobulin, buffered aqueous solution
SAB1400206
Monoclonal Anti-PGR antibody produced in mouse, clone 5D10, purified immunoglobulin, buffered aqueous solution
SAB1400207
Monoclonal Anti-PGR antibody produced in mouse, clone 4E9, purified immunoglobulin, buffered aqueous solution
SAB1404196
Monoclonal Anti-PGR, (N-terminal) antibody produced in mouse, clone 2C2, purified immunoglobulin, buffered aqueous solution