EMAIL THIS PAGE TO A FRIEND

The Journal of investigative dermatology

Melanoma Cell Galectin-1 Ligands Functionally Correlate with Malignant Potential.


PMID 25756799

Abstract

Galectin-1 (Gal-1)-binding to Gal-1 ligands on immune and endothelial cells can influence melanoma development through dampening antitumor immune responses and promoting angiogenesis. However, whether Gal-1 ligands are functionally expressed on melanoma cells to help control intrinsic malignant features remains poorly understood. Here, we analyzed expression, identity, and function of Gal-1 ligands in melanoma progression. Immunofluorescent analysis of benign and malignant human melanocytic neoplasms revealed that Gal-1 ligands were abundant in severely dysplastic nevi, as well as in primary and metastatic melanomas. Biochemical assessments indicated that melanoma cell adhesion molecule (MCAM) was a major Gal-1 ligand on melanoma cells that was largely dependent on its N-glycans. Other melanoma cell Gal-1 ligand activity conferred by O-glycans was negatively regulated by α2,6 sialyltransferase ST6GalNAc2. In Gal-1-deficient mice, MCAM-silenced (MCAM(KD)) or ST6GalNAc2-overexpressing (ST6(O/E)) melanoma cells exhibited slower growth rates, underscoring a key role for melanoma cell Gal-1 ligands and host Gal-1 in melanoma growth. Further analysis of MCAM(KD) or ST6(O/E) melanoma cells in cell migration assays indicated that Gal-1 ligand-dependent melanoma cell migration was severely inhibited. These findings provide a refined perspective on Gal-1/melanoma cell Gal-1 ligand interactions as contributors to melanoma malignancy.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

N9389
4-Nitrophenyl phosphate disodium salt hexahydrate, tablet
C6H4NNa2O6P · 6H2O
N2765
4-Nitrophenyl phosphate disodium salt hexahydrate, tablet
C6H4NNa2O6P · 6H2O
N2640
4-Nitrophenyl phosphate disodium salt hexahydrate, tablet
C6H4NNa2O6P · 6H2O
N4645
4-Nitrophenyl phosphate disodium salt hexahydrate, powder, BioReagent, suitable for cell culture, ≥97%
C6H4NNa2O6P · 6H2O
71768
4-Nitrophenyl phosphate disodium salt hexahydrate, for enzyme immunoassay, ≥99.0% (enzymatic)
C6H4NNa2O6P · 6H2O
SAB1406108
Anti-MCAM antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA008848
Anti-MCAM antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2700812
Anti-MCAM antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB2701024
Anti-MCAM antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB1409701
Anti-SOX10 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
S8193 Anti-Sox10 antibody produced in rabbit, affinity isolated antibody, ammonium sulfate suspension
SAB2106297
Anti-SOX10 antibody produced in rabbit, affinity isolated antibody, lyophilized powder
SAB2105400
Anti-SOX10, (N-terminal) antibody produced in rabbit, affinity isolated antibody
SAB1402361
Monoclonal Anti-SOX10 antibody produced in mouse, clone 1E6, purified immunoglobulin, buffered aqueous solution
P4744
Phosphatase substrate, powder
C6H4NNa2O6P · 6H2O
S0942
Phosphatase substrate, 5 mg tablets
C6H4NNa2O6P · 6H2O
P5994
Phosphatase substrate, 40 mg tablets
C6H4NNa2O6P · 6H2O
P5744
Phosphatase substrate, 40 mg capsules
C6H4NNa2O6P · 6H2O
P5869
Phosphatase substrate, 100 mg capsules
C6H4NNa2O6P · 6H2O
SRE0026
Phosphatase substrate, Suitable for manufacturing of diagnostic kits and reagents
C6H4NNa2O6P · 6H2O
V4512 Vascular Endothelial Growth Factor from mouse, VEGF, recombinant, expressed in E. coli, powder, suitable for cell culture