EMAIL THIS PAGE TO A FRIEND

Biochemical and biophysical research communications

YY1 positively regulates human UBIAD1 expression.


PMID 25772619

Abstract

Vitamin K is involved in bone formation and blood coagulation. Natural vitamin K compounds are composed of the plant form phylloquinone (vitamin K1) and a series of bacterial menaquionones (MK-n; vitamin K2). Menadione (vitamin K3) is an artificial vitamin K compound. MK-4 contains 4-isoprenyl as a side group in the 2-methyl-1,4-naphthoquinone common structure and has various bioactivities. UbiA prenyltransferase domain containing 1 (UBIAD1 or TERE1) is the menaquinone-4 biosynthetic enzyme. UBIAD1 transcript expression significantly decreases in patients with prostate carcinoma and overexpressing UBIAD1 inhibits proliferation of a tumour cell line. UBIAD1 mRNA expression is ubiquitous in mouse tissues, and higher UBIAD1 mRNA expression levels are detected in the brain, heart, kidneys and pancreas. Several functions of UBIAD1 have been reported; however, regulation of the human UBIAD1 gene has not been elucidated. Here we report cloning and characterisation of the human UBIAD1 promoter. A 5' rapid amplification of cDNA ends analysis revealed that the main transcriptional start site was 306 nucleotides upstream of the translation initiation codon. Deletion and mutation analyses revealed the functional importance of the YY1 consensus motif. Electrophoretic gel mobility shift and chromatin immunoprecipitation assays demonstrated that YY1 binds the UBIAD1 promoter in vitro and in vivo. In addition, YY1 small interfering RNA decreased endogenous UBIAD1 mRNA expression and UBIAD1 conversion activity. These results suggest that YY1 up-regulates UBIAD1 expression and UBIAD1 conversion activity through the UBIAD1 promoter.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

AV38301
Anti-YY1 (AB1) antibody produced in rabbit, affinity isolated antibody
AV100898
Anti-YY1 (AB2) antibody produced in rabbit, affinity isolated antibody
AV100899 Anti-YY1 (AB3) antibody produced in rabbit, affinity isolated antibody
SAB4200303
Anti-YY1 (N-terminal) antibody produced in rabbit, Ig fraction of antiserum
SAB1406605
Anti-YY1 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
SAB1412082
ANTI-YY1 antibody produced in mouse, clone 4D2, purified immunoglobulin, buffered aqueous solution
SAB1412083
ANTI-YY1 antibody produced in mouse, clone 2C5, purified immunoglobulin, buffered aqueous solution
SAB1412084
ANTI-YY1 antibody produced in mouse, clone 4C1, purified immunoglobulin, buffered aqueous solution
HPA001119
Anti-YY1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB4200302
Anti-YY1 antibody produced in rabbit, IgG fraction of antiserum
EHU043571 MISSION® esiRNA, esiRNA human YY1 (esiRNA1)
WH0007528M1
Monoclonal Anti-YY1 antibody produced in mouse, clone 2C4, purified immunoglobulin, buffered aqueous solution
SAB1404533
Monoclonal Anti-YY1 antibody produced in mouse, clone 4A5, purified immunoglobulin, buffered aqueous solution
PLA0268
Rabbit anti-YY1 Antibody, Affinity Purified, Powered by Bethyl Laboratories, Inc.