EMAIL THIS PAGE TO A FRIEND

Parasitology research

Neurotoxocarosis alters myelin protein gene transcription and expression.


PMID 25773181

Abstract

Neurotoxocarosis is an infection of the central nervous system caused by migrating larvae of the common dog and cat roundworms (Toxocara canis and Toxocara cati), which are zoonotic agents. As these parasites are prevalent worldwide and neuropathological and molecular investigations on neurotoxocarosis are scare, this study aims to characterise nerve fibre demyelination associated with neurotoxocarosis on a molecular level. Transcription of eight myelin-associated genes (Cnp, Mag, Mbp, Mog, Mrf-1, Nogo-A, Plp1, Olig2) was determined in the mouse model during six time points of the chronic phase of infection using qRT-PCR. Expression of selected proteins was analysed by Western blotting or immunohistochemistry. Additionally, demyelination and neuronal damage were investigated histologically. Significant differences (p ≤ 0.05) between transcription rates of T. canis-infected and uninfected control mice were detected for all analysed genes while T. cati affected five of eight investigated genes. Interestingly, 2', 3 ´-cyclic nucleotide 3'-phosphodiesterase (Cnp) and myelin oligodendrocyte glycoprotein (Mog) were upregulated in both T. canis- and T. cati-infected mice preceding demyelination. Later, CNPase expression was additionally enhanced. As expected, myelin basic protein (Mbp) was downregulated in cerebra and cerebella of T. canis-infected mice when severe demyelination was present 120xa0days post infectionem (dpi). The transcriptional pattern observed in the present study appears to reflect direct traumatic and hypoxic effects of larval migration as well as secondary processes including host immune reactions, demyelination and attempts to remyelinate damaged areas.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB4300464
Anti-APP (Ab-668) antibody produced in rabbit, affinity isolated antibody
SAB2100134
Anti-APP (ab1) antibody produced in rabbit, affinity isolated antibody
SAB2100135
Anti-APP (ab2) antibody produced in rabbit, affinity isolated antibody
SAB2100136
Anti-APP (ab3) antibody produced in rabbit, IgG fraction of antiserum
SAB2502012
Anti-APP (N-terminal) antibody produced in goat, affinity isolated antibody, buffered aqueous solution
HPA001462
Anti-APP antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB4200535
Anti-APP antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody
SAB3500274
Anti-APP antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB5200113
Anti-APP antibody produced in rabbit, 1 mg/mL, affinity isolated antibody
SAB2107345
Anti-APP antibody produced in rabbit, affinity isolated antibody
SAB2106270
Anti-APP antibody produced in rabbit, affinity isolated antibody
SAB1405637
Anti-CNP antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
AV33880
Anti-CNP antibody produced in rabbit, IgG fraction of antiserum
HPA023266
Anti-CNP antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab1
HPA023278
Anti-CNP antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab2
HPA023280
Anti-CNP antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab3
HPA023338
Anti-CNP antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab4
SAB2100458
Anti-CNP antibody produced in rabbit, affinity isolated antibody
C9743 Anti-CNPase antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
SAB2104172
Anti-MBP antibody produced in rabbit, affinity isolated antibody
HPA049222
Anti-MBP antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
M3821 Anti-Myelin Basic Protein (MBP) antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
AV32753
Anti-OLIG2 (AB1) antibody produced in rabbit, affinity isolated antibody
AV31464
Anti-OLIG2 (AB2) antibody produced in rabbit, affinity isolated antibody
AV32752
Anti-OLIG2 (AB3) antibody produced in rabbit, affinity isolated antibody
SAB1412293
ANTI-OLIG2 antibody produced in mouse, clone 2A8, purified immunoglobulin, buffered aqueous solution
SAB1412294
ANTI-OLIG2 antibody produced in mouse, clone 2B11, purified immunoglobulin, buffered aqueous solution
HPA003254
Anti-OLIG2 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AV46812
Anti-RTN4 (AB1) antibody produced in rabbit, affinity isolated antibody
AV46813
Anti-RTN4 (AB2) antibody produced in rabbit, affinity isolated antibody