EMAIL THIS PAGE TO A FRIEND

The Journal of reproduction and development

Status of autophagy, lysosome activity and apoptosis during corpus luteum regression in cattle.


PMID 25819401

Abstract

Corpus luteum (CL) regression is required during the estrous cycle. During CL regression, luteal cells stop producing progesterone and are degraded by apoptosis. However, the detailed mechanism of CL regression in cattle has not been fully elucidated. The aim of this study was to evaluate autophagy, lysosome activity, and apoptosis during CL regression in cattle. The expression of autophagy-related genes (LC3α, LC3β, Atg3, and Atg7) and the protein LC3-II was significantly higher in the late CL than in the mid CL. In addition, autophagy activity was significantly increased in the late CL. Moreover, gene expression of the autophagy inhibitor mammalian target of rapamycin (mTOR) was significantly lower in the late CL than in the mid CL. Lysosome activation and expression of cathepsin-related genes (CTSB, CTSD, and CTSZ) showed significant increases in the late CL and were associated with an increase in cathepsin B protein. In addition, mRNA expression and activity of caspase 3 (CASP3), an apoptotic enzyme, were significantly higher in the late CL than in the mid CL. These results suggest simultaneous upregulation of autophagy-related factors, lysosomal enzymes and apoptotic mediators, which are involved in regression of the bovine CL.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

C6243
Anti-Cathepsin B antibody, Mouse monoclonal, clone CB59-4B11, purified from hybridoma cell culture
SAB1405676
Anti-CTSB antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA018156
Anti-CTSB antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB3701207 Anti-Mouse IgM (μ-chain specific), F(ab′)2 fragment-Alkaline Phosphatase antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB3701208 Anti-Mouse IgM (μ-chain specific), F(ab′)2 fragment-Biotin antibody produced in goat, affinity isolated antibody, lyophilized powder
SAB3701205 Anti-Mouse IgM (μ-chain specific), F(ab′)2 fragment-Fluorescein antibody produced in goat, affinity isolated antibody, lyophilized powder
A4540 Anti-Mouse IgM (μ-chain specific)−Agarose antibody produced in goat, IgG fraction of antiserum, saline suspension
A9688 Anti-Mouse IgM (μ-chain specific)–Alkaline Phosphatase antibody produced in goat, affinity isolated antibody, buffered aqueous glycerol solution
B9265
Anti-Mouse IgM (μ-chain specific)–Biotin antibody produced in goat, affinity isolated antibody, buffered aqueous solution
F9259 Anti-Mouse IgM (μ-chain specific)–FITC antibody produced in goat, affinity isolated antibody, buffered aqueous solution
A8786
Anti-Mouse IgM (μ-chain specific)–Peroxidase antibody produced in goat, affinity isolated antibody, buffered aqueous solution