EMAIL THIS PAGE TO A FRIEND

Journal of cellular physiology

Nuclear Translocation of Calpain-2 Mediates Apoptosis of Hypertrophied Cardiomyocytes in Transverse Aortic Constriction Rat.


PMID 25820375

Abstract

Apoptosis of cardiomyocytes plays an important role in the transition from cardiac hypertrophy to heart failure. Hypertrophied cardiomyocytes show enhanced susceptibility to apoptosis. Therefore, the aim of this study was to determine the susceptibility to apoptosis and its mechanism in hypertrophied cardiomyocytes using a rat model of transverse abdominal aortic constriction (TAC). Sixteen weeks of TAC showed compensatory and pathological hypertrophy in the left ventricle. TUNEL-positive nuclei were significantly increased in TAC with angiotensin II (Ang II) treatment. Calpain inhibitor, PD150606, effectively inhibited Ang II-induced apoptosis of hypertrophied cardiomyocytes. Ang II increased nuclear translocation of intracellular Ca(2+) activated calpain-2 in hypertrophied cardiomyocytes. Ang II enhanced the interaction between activated calpain-2 and Ca(2+)/calmodulin-dependent protein kinase II δB (CaMKIIδB), and promoted the degradation of CaMKIIδB by calpain-2 in the nuclei of hypertrophied cardiomyocytes. Consequently, the depressed CaMKIIδB downregulated the expression of antiapoptotic Bcl-2 leading to mitochondrial depolarization and release of cytochrome c led to apoptosis of hypertrophied cardiomyocytes. In conclusion, hypertrophied cardiomyocytes show increased susceptibility to apoptosis during Ang II stimulation via nuclear calpain-2 and CaMKIIδB pathway.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

GW20006A Anti-Calpastatin antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
SAB2501257
Anti-CAMK2A antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB1405553
Anti-CAMK2A antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
SAB1405558
Anti-CAST antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
SAB2502100
Anti-LMNB1 antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB2502101
Anti-LMNB1 antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB2101352
Anti-LMNB1 antibody produced in rabbit, affinity isolated antibody
SAB1410759
Anti-LMNB1 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
C270 Monoclonal Anti-Calpastatin antibody produced in mouse, clone 1F7E3D10, ascites fluid, buffered aqueous solution
WH0000815M1
Monoclonal Anti-CAMK2A antibody produced in mouse, clone 2C4, purified immunoglobulin, buffered aqueous solution
SAB1403629
Monoclonal Anti-CAMK2A antibody produced in mouse, clone 2C12, purified immunoglobulin, buffered aqueous solution
SAB4100450
Monoclonal Anti-CAST antibody produced in mouse, clone CAST-A387, culture supernatant
SAB1402139
Monoclonal Anti-CAST antibody produced in mouse, clone 2C5, purified immunoglobulin, buffered aqueous solution
SAB1400153
Monoclonal Anti-LMNB1 antibody produced in mouse, clone 4B10, purified immunoglobulin, buffered aqueous solution
SAB1404027
Monoclonal Anti-LMNB1 antibody produced in mouse, clone 4E4, purified immunoglobulin, buffered aqueous solution