EMAIL THIS PAGE TO A FRIEND

Molecular medicine reports

Role of calcium in the regulation of bone morphogenetic protein 2, runt-related transcription factor 2 and Osterix in primary renal tubular epithelial cells by the vitamin D receptor.


PMID 25823394

Abstract

The aim of the present study was to investigate the effect of 1,25(OH)2D3/vitamin D receptor (VDR) and calcium on the expression levels of osteogenic factors in primary renal tubular epithelial cells (RTECs) using genetic hypercalciuric rats. The basal levels of osteogenic factors were detected in Sprague Dawley and genetic hypercalciuric rats. The gene and protein levels of bone morphogenetic protein 2 (BMP2), runt-related transcription factor 2 (Runx2) and osterix were detected in the RTECs transduced with Lenti-VDR-sh and were incubated with calcium. Using the o-cresolphthalein complexone method, the calcium levels of the primary RTECs cultured with Lenti-VDR-sh and with 1,25(OH)2D3 were assessed. The basal levels of BMP2, Runx2 and Osterix in the cells were significantly higher in the genetic hypercalciuric rats compared with the control rats. VDR knockdown in the RTECs reduced the expression levels of BMP2, Runx2 and Osterix. The calcium depositions in the primary RTECs were also decreased following exposure to Lenti-VDR-sh, but increased following treatment with 1,25(OH)2D3. The expression levels of BMP2, Runx2 and Osterix were markedly increased in the cells incubated with calcium compared with the cells treated with normal saline and the untreated cells. These findings indicated that osteogenic factors, including BMP2, Runx2 and Osterix may be important in renal stone formation in idiopathic hypercalciuria. VDR may mediate the increased expression levels of BMP2, Runx2 and Osterix by positively regulating calcium levels in primary RTECs.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB2106696
Anti-BMP2 antibody produced in rabbit, affinity isolated antibody, lyophilized powder
SAB1411278
Anti-BMP2 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
AV36678
Anti-RUNX2 (AB1) antibody produced in rabbit, IgG fraction of antiserum
AV36679
Anti-RUNX2 (AB2) antibody produced in rabbit, IgG fraction of antiserum, lyophilized powder
SAB1412189
ANTI-RUNX2 antibody produced in mouse, clone 6E1, purified immunoglobulin, buffered aqueous solution
SAB1412247
ANTI-RUNX2 antibody produced in mouse, clone 4E5, ascites fluid, buffered aqueous solution
SAB1412248
ANTI-RUNX2 antibody produced in mouse, clone 4D7, purified immunoglobulin, buffered aqueous solution
SAB1412249
ANTI-RUNX2 antibody produced in mouse, clone 4B4, purified immunoglobulin, buffered aqueous solution
SAB1412665
ANTI-RUNX2 antibody produced in mouse, clone 2B9, purified immunoglobulin, buffered aqueous solution
SAB1412666
ANTI-RUNX2 antibody produced in mouse, clone 1D2, purified immunoglobulin, buffered aqueous solution
SAB1412667
ANTI-RUNX2 antibody produced in mouse, clone 3F5, purified immunoglobulin, buffered aqueous solution
SAB1412668
ANTI-RUNX2 antibody produced in mouse, clone 4C8, purified immunoglobulin, buffered aqueous solution
HPA022040
Anti-RUNX2 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2106220
Anti-RUNX2 antibody produced in rabbit, affinity isolated antibody
AV31622
Anti-SP7 antibody produced in rabbit, affinity isolated antibody
SAB2104296
Anti-SP7, (N-terminal) antibody produced in rabbit, affinity isolated antibody
WH0000650M2 Monoclonal Anti-BMP2 antibody produced in mouse, clone 1A11, purified immunoglobulin, buffered aqueous solution
SAB1403609 Monoclonal Anti-BMP2, (C-terminal) antibody produced in mouse, clone 4B12, purified immunoglobulin, buffered aqueous solution
B9553 Monoclonal Anti-Bone Morphogenetic Protein 2 antibody produced in mouse, clone 65529.111, purified immunoglobulin, lyophilized powder
WH0000860M1
Monoclonal Anti-RUNX2 antibody produced in mouse, clone 1D8, purified immunoglobulin, buffered aqueous solution
AMAB90591
Monoclonal Anti-RUNX2 antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL0232, purified immunoglobulin, buffered aqueous glycerol solution
SAB1403638
Monoclonal Anti-RUNX2 antibody produced in mouse, clone 4D5, purified immunoglobulin, buffered aqueous solution
AMAB90594
Monoclonal Anti-RUNX2 antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL0235, purified immunoglobulin, buffered aqueous glycerol solution
WH0121340M1
Monoclonal Anti-SP7 antibody produced in mouse, clone 2G6, purified immunoglobulin, buffered aqueous solution
SAB1405350
Monoclonal Anti-SP7 antibody produced in mouse, clone 1C11, purified immunoglobulin, buffered aqueous solution
P5631
o-Cresolphthalein Complexone, powder
C32H32N2O12