EMAIL THIS PAGE TO A FRIEND

Biochimica et biophysica acta

Involvement of activated SUMO-2 conjugation in cardiomyopathy.


PMID 25857621

Abstract

Sumoylation is a posttranslational modification that regulates a wide spectrum of cellular activities. Cardiomyopathy is the leading cause of heart failure. Whether sumoylation, particularly SUMO-2/3 conjugation, is involved in cardiomyopathy has not been investigated. We report here that SUMO-2/3 conjugation was elevated in the human failing hearts, and we investigated the impact of increased SUMO-2 conjugation on heart function by using the gain-of-function approach in mice, in which cardiac specific expression of constitutively active SUMO-2 was governed by alpha myosin heavy chain promoter (MHC-SUMO-2 transgenic, SUMO-2-Tg). Four of five independent SUMO-2-Tg mouse lines exhibited cardiomyopathy with various severities, ranging from acute heart failure leading to early death to the development of chronic cardiomyopathy with aging. We further revealed that SUMO-2 directly regulated apoptotic process by at least partially targeting calpain 2 and its natural inhibitor calpastatin. SUMO conjugation to calpain 2 promoted its enzymatic activity, and SUMO attachment to calpastatin mainly promoted its turnover and altered its subcellular distribution. Thus, enhanced SUMO-2 conjugation led to increased apoptosis and played a pathogenic role in the development of cardiomyopathy and heart failure.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

GW20006A Anti-Calpastatin antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
HPA024470
Anti-CAPN2 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1411480
Anti-CAPN2 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
SAB1405558
Anti-CAST antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
S5446
Anti-SUMO-1 (C-terminal) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
S8070
Anti-SUMO-1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
S9571
Anti-SUMO-2 antibody produced in rabbit, ~0.6 mg/mL, affinity isolated antibody, buffered aqueous solution
SAB2106210
Anti-SUMO1 antibody produced in rabbit, affinity isolated antibody
SAB1301315 ANTI-SUMO2 (C-TERM) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
SAB4502824
Anti-SUMO2 (Cleaved-Gly93) antibody produced in rabbit, affinity isolated antibody
SAB2701779
Anti-sumo2 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB1401366
Anti-SUMO2 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
C270 Monoclonal Anti-Calpastatin antibody produced in mouse, clone 1F7E3D10, ascites fluid, buffered aqueous solution
SAB4100449
Monoclonal Anti-CAPN2 antibody produced in mouse, clone CAPN2-A386, culture supernatant
SAB4100450
Monoclonal Anti-CAST antibody produced in mouse, clone CAST-A387, culture supernatant
SAB1402139
Monoclonal Anti-CAST antibody produced in mouse, clone 2C5, purified immunoglobulin, buffered aqueous solution
SAB1402954
Monoclonal Anti-SUMO1 antibody produced in mouse, clone 3D7, purified immunoglobulin, buffered aqueous solution
SAB1404385
Monoclonal Anti-SUMO2 antibody produced in mouse, clone 2C7-1A11, purified immunoglobulin, buffered aqueous solution
SAB1402359
Monoclonal Anti-SUMO2 antibody produced in mouse, clone 2H8, purified immunoglobulin, buffered aqueous solution