EMAIL THIS PAGE TO A FRIEND

Clinical biochemistry

Possible involvement of sphingomyelin in the regulation of the plasma sphingosine 1-phosphate level in human subjects.


PMID 25863111

Abstract

Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid mediator. Although the plasma S1P concentration is reportedly determined by cellular components, including erythrocytes, platelets, and vascular endothelial cells, the possible involvement of other factors, such as serum sphingomyelin (SM) and autotaxin (ATX), remains to be elucidated. We measured S1P using high-performance liquid chromatography (HPLC), SM and lysophosphatidic acid (LPA) using enzymatic assays, ATX antigen using a two-site enzyme immunoassay, and ATX activity using a lysophospholipase D activity assay. To fractionate the lipoproteins, plasma samples were separated using fast protein liquid chromatography (FPLC) utilizing a Superose 6 column. The plasma S1P level was positively correlated with the levels of SM and lysophosphatidylcholine, but not with the level of phosphatidylcholine. Although SM was present in the very low-density lipoprotein (VLDL) fraction, neither the plasma S1P level nor the SM level was affected by feeding. The plasma S1P level was negatively correlated with the ATX activity. Although the incubation of 100 μmol/L of sphingosylphosphorylcholine (SPC) with the serum resulted in a significant increase in the S1P level because of the presence of ATX, the physiological concentration of SPC did not mimic this effect. The plasma S1P level was affected by the serum SM level, while the possibility of ATX involvement in the increase in the plasma S1P level was considered to be remote at least in healthy human subjects.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB1412735
Anti-ENPP2 antibody produced in mouse, purified immunoglobulin, 1 mg/mL
SAB2700495
Anti-ENPP2 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB2700320
Anti-ENPP2 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB1412736
Anti-ENPP2 antibody produced in rabbit, purified immunoglobulin, 1 mg/mL
SAB2105612
Anti-ENPP2, (N-terminal) antibody produced in rabbit, affinity isolated antibody
SAB1412284
ANTI-SMG1 antibody produced in mouse, clone 1C12, purified immunoglobulin, buffered aqueous solution
SAB1412637
ANTI-SMG1 antibody produced in mouse, clone 2B10, purified immunoglobulin, buffered aqueous solution
SAB1412638
ANTI-SMG1 antibody produced in mouse, clone 4E4, purified immunoglobulin, buffered aqueous solution
HPA006870 Anti-SMG1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
D3439
D-erythro-Dihydrosphingosine 1-phosphate, ≥98% (TLC), waxy solid
C18H40NO5P
D9766
Dipyridamole, ≥98% (TLC), powder
C24H40N8O4
WH0023049M1
Monoclonal Anti-SMG1 antibody produced in mouse, clone 1G5, purified immunoglobulin, buffered aqueous solution
SAB1404950
Monoclonal Anti-SMG1 antibody produced in mouse, clone 1A8, purified immunoglobulin, buffered aqueous solution
735914
Octylphosphonic acid, 97%
C8H19O3P
P0657
Phthaldialdehyde, suitable for HPLC fluorimetric detection of amino acids, ≥99% (HPLC), powder
C8H6O2
P1378
Phthaldialdehyde, ≥97% (HPLC), powder (may contain lumps)
C8H6O2
79760
Phthaldialdehyde, for fluorescence, ≥99.0% (HPLC)
C8H6O2
PLA0194
Rabbit anti-SMG1 Antibody, Affinity Purified, Powered by Bethyl Laboratories, Inc.