EMAIL THIS PAGE TO A FRIEND

Biomaterials

Integration of donor mesenchymal stem cell-derived neuron-like cells into host neural network after rat spinal cord transection.


PMID 25890718

Abstract

Functional deficits following spinal cord injury (SCI) primarily attribute to loss of neural connectivity. We therefore tested if novel tissue engineering approaches could enable neural network repair that facilitates functional recovery after spinal cord transection (SCT). Rat bone marrow-derived mesenchymal stem cells (MSCs), genetically engineered to overexpress TrkC, receptor of neurotrophin-3 (NT-3), were pre-differentiated into cells carrying neuronal features via co-culture with NT-3 overproducing Schwann cells in 3-dimensional gelatin sponge (GS) scaffold for 14 days inxa0vitro. Intra-GS formation of MSC assemblies emulating neural network (MSC-GS) were verified morphologically via electron microscopy (EM) and functionally by whole-cell patch clamp recording of spontaneous post-synaptic currents. The differentiated MSCs still partially maintained prototypic property with the expression of some mesodermal cytokines. MSC-GS or GS was then grafted acutely into a 2xa0mm-wide transection gap in the T9-T10 spinal cord segments of adult rats. Eight weeks later, hindlimb function of the MSC-GS-treated SCT rats was significantly improved relative to controls receiving the GS or lesion only as indicated by BBB score. The MSC-GS transplantation also significantly recovered cortical motor evoked potential (CMEP). Histologically, MSC-derived neuron-like cells maintained their synapse-like structures inxa0vivo; they additionally formed similar connections with host neurites (i.e., mostly serotonergic fibers plus a few corticospinal axons; validated by double-labeled immuno-EM). Moreover, motor cortex electrical stimulation triggered c-fos expression in the grafted and lumbar spinal cord cells of the treated rats only. Our data suggest that MSC-derived neuron-like cells resulting from NT-3-TrkC-induced differentiation can partially integrate into transected spinal cord and this strategy should be further investigated for reconstructing disrupted neural circuits.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

324817
(Aminomethyl)phosphonic acid, 99%
CH6NO3P
345571
1,5,7-Triazabicyclo[4.4.0]dec-5-ene, 98%
C7H13N3
SAB1401024
Anti-ATP6V1E1 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
HPA029196
Anti-ATP6V1E1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
F7799
Anti-c-Fos antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
F137 Anti-c-Fos antibody produced in rabbit, IgG fraction of antiserum, lyophilized powder
HPA018531
Anti-C-FOS antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1306144
ANTI-C-FOS(C-TERMINAL) antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
HPA026481
Anti-DBT antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab1
HPA026485
Anti-DBT antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab2
HPA026533
Anti-DBT antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab3
SAB2104091
Anti-DBT, (N-terminal) antibody produced in rabbit, affinity isolated antibody
AV41994
Anti-DLAT antibody produced in rabbit, IgG fraction of antiserum
HPA040786
Anti-DLAT antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1410301
Anti-DLAT antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
SAB2701694
Anti-dlst antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
HPA003010
Anti-DLST antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1401116
Anti-DLST antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
SAB2701135
Anti-DLST antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB2100832
Anti-FOS (ab1) antibody produced in rabbit, affinity isolated antibody
SAB2100833
Anti-FOS (ab2) antibody produced in rabbit, affinity isolated antibody
AV100639
Anti-FOS antibody produced in rabbit, IgG fraction of antiserum
SAB2104185
Anti-FOS antibody produced in rabbit, affinity isolated antibody
SAB4500995
Anti-FOS antibody produced in rabbit, affinity isolated antibody
SAB4500993
Anti-FOS, C-Terminal antibody produced in rabbit, affinity isolated antibody
SAB4500996
Anti-FOS, C-Terminal antibody produced in rabbit, affinity isolated antibody
SAB1409383
Anti-NTRK3 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
SAB5300229
Monoclonal Anti-NTRK3 antibody produced in mouse, clone 7H6, ascites fluid
SAB5300230
Monoclonal Anti-NTRK3 antibody produced in mouse, clone 4G5, ascites fluid
SAB1402294
Monoclonal Anti-NTRK3 antibody produced in mouse, clone 4D5, purified immunoglobulin, buffered aqueous solution