EMAIL THIS PAGE TO A FRIEND

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Ssb2/Nabp1 is dispensable for thymic maturation, male fertility, and DNA repair in mice.


PMID 25917330

Abstract

SSB1 and SSB2 are newly identified single-stranded (ss) DNA binding proteins that play a crucial role in genome maintenance in humans. We recently generated a knockout mouse model of Ssb1 and revealed its essential role for neonatal survival. Notably, we found compensatory up-regulation of Ssb2 protein levels in multiple tissues of conditional Ssb1(-/-) mice, suggesting functional compensation between these 2 proteins. We report here the first description of Ssb2(-/-) knockout mice. Surprisingly, unlike Ssb1 knockout mice, Ssb2(-/-) mice are viable and fertile and do not exhibit marked phenotypic changes when compared with their Ssb2(+/+) and Ssb2(+/-) littermates. Notably, we did not detect any pathologic changes in the thymus, spleen, or testes, tissues with the most abundant expression of Ssb2. Moreover, Ssb2(-/-) mouse embryonic fibroblasts (MEFs) did not show any sensitivity to DNA-damaging agents, or defects in DNA repair capacity. However, we observed modest up-regulation of Ssb1 levels in Ssb2(-/-) MEFs as well as in Ssb2(-/-) thymus and spleen, suggesting that Ssb1 is likely able to compensate for the loss of Ssb2 in mice. Altogether, our results show that Ssb2 is dispensable for embryogenesis and adult tissue homeostasis, including thymopoiesis, splenic development, male fertility, and DNA repair in mice.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB4300390
Anti-CHEK1 (Ab-317) antibody produced in rabbit, affinity isolated antibody
AV32589
Anti-CHEK1 antibody produced in rabbit, affinity isolated antibody
SAB2104134
Anti-CHEK1 antibody produced in rabbit, affinity isolated antibody
GW21552 Anti-CHEK2 antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
SAB1407116
Anti-CHEK2 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA001878
Anti-CHEK2 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AV30290
Anti-CHEK2 antibody produced in rabbit, affinity isolated antibody
SAB2100415
Anti-CHEK2 antibody produced in rabbit, affinity isolated antibody
SAB4500207
Anti-CHK1 antibody produced in rabbit, affinity isolated antibody
SAB4500208
Anti-CHK1 antibody produced in rabbit, affinity isolated antibody
SAB4500209
Anti-CHK1 antibody produced in rabbit, affinity isolated antibody
SAB4500210
Anti-CHK1 antibody produced in rabbit, affinity isolated antibody
SAB4500211
Anti-CHK1 antibody produced in rabbit, affinity isolated antibody
SAB3500192
Anti-Chk2 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
C9108 Anti-Chk2 antibody, Mouse monoclonal, clone DCS-273, purified from hybridoma cell culture
C9233
Anti-Chk2 antibody, Mouse monoclonal, clone DCS-270, purified from hybridoma cell culture
SAB4300116
Anti-phospho-CHEK1 (pSer280) antibody produced in rabbit, affinity isolated antibody
SAB4504363
Anti-phospho-Chk1 (pSer280) antibody produced in rabbit, affinity isolated antibody
SAB4504146
Anti-phospho-Chk1 (pSer286) antibody produced in rabbit, affinity isolated antibody
SAB4504025
Anti-phospho-Chk1 (pSer296) antibody produced in rabbit, affinity isolated antibody
SAB4504147
Anti-phospho-Chk1 (pSer301) antibody produced in rabbit, affinity isolated antibody
SAB4504364
Anti-phospho-Chk1 (pSer317) antibody produced in rabbit, affinity isolated antibody
SAB4504365
Anti-phospho-Chk1 (pSer345) antibody produced in rabbit, affinity isolated antibody
SAB4504499
Anti-phospho-p53 (pSer15) antibody produced in rabbit, affinity isolated antibody
SAB4503953
Anti-phospho-p53 (pSer20) antibody produced in rabbit, affinity isolated antibody
SAB4504500
Anti-phospho-p53 (pSer315) antibody produced in rabbit, affinity isolated antibody
SAB4504501
Anti-phospho-p53 (pSer33) antibody produced in rabbit, affinity isolated antibody
SAB4504592
Anti-phospho-p53 (pSer366) antibody produced in rabbit, affinity isolated antibody
SAB4504502
Anti-phospho-p53 (pSer37) antibody produced in rabbit, affinity isolated antibody
SAB4504593
Anti-phospho-p53 (pSer378) antibody produced in rabbit, affinity isolated antibody