EMAIL THIS PAGE TO A FRIEND

Cell cycle (Georgetown, Tex.)

A dynein independent role of Tctex-1 at the kinetochore.


PMID 25928583

Abstract

Dynein light chains are accessory subunits of the cytoplasmic dynein complex, a minus-end directed microtubule motor. Here, we demonstrate that the dynein light chain Tctex-1 associates with unattached kinetochores and is essential for accurate chromosome segregation. Tctex-1 knockdown in cells does not affect the localization and function of dynein at the kinetochore, but produces a prolonged mitotic arrest with a few misaligned chromosomes, which are subsequently missegregated during anaphase. This function is independent of Tctex-1's association with dynein. The kinetochore localization of Tctex-1 is independent of the ZW10-dynein pathway, but requires the Ndc80 complex. Thus, our findings reveal a dynein independent role of Tctex-1 at the kinetochore to enhance the stability of kinetochore-microtubule attachment.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB1301499
ANTI-ANKS1B (CENTER) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
C7488
Anti-CENP-E antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
HPA042294
Anti-CENPE antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1306849
ANTI-CENPE(C-TERMINAL) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB2501289
Anti-CLIP1 (C-terminal) antibody produced in goat, affinity isolated antibody, buffered aqueous solution
HPA026678
Anti-CLIP1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB4502704
Anti-CLIP1, C-Terminal antibody produced in rabbit, affinity isolated antibody
PRS4659
Anti-CLIP170 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB1303595
ANTI-COX7A2L (CENTER) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
SAB1406787
Anti-COX7A2L antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
SAB1409980
Anti-COX7A2L antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
E3406
Anti-EB1 antibody produced in rabbit, ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
SAB4503302
Anti-MAD1 antibody produced in rabbit, affinity isolated antibody
HPA003635
Anti-MAD1L1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA003600
Anti-MAPRE1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2107652
Anti-MAPRE1 antibody produced in rabbit, affinity isolated antibody
HPA016738
Anti-MAPRE2 antibody produced in rabbit, Ab2, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA016739
Anti-MAPRE2 antibody produced in rabbit, Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA001599
Anti-MXD1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AV38141
Anti-MXD1 antibody produced in rabbit, affinity isolated antibody, lyophilized powder
SAB2106429
Anti-MXD1 antibody produced in rabbit, affinity isolated antibody
SAB2105310
Anti-MXD1, (C-terminal) antibody produced in rabbit, affinity isolated antibody, lyophilized powder
D125407
DIC, 99%
C7H14N2
EHU096721 MISSION® esiRNA, esiRNA human CLIP1 (esiRNA1)
SAB3701429
Monoclonal Anti-CENP-E antibody produced in mouse, clone 1H12, purified immunoglobulin
D9944
Monoclonal Anti-DYNLT1 antibody produced in mouse, ~1.0 mg/mL, clone T1, purified immunoglobulin, buffered aqueous solution
M8069 Monoclonal Anti-Mad1 antibody produced in mouse, ~2 mg/mL, clone 9B10, purified immunoglobulin, buffered aqueous solution
SAB3701452 Monoclonal Anti-MAD1L1 antibody produced in mouse, clone 9B10, purified immunoglobulin
SAB1403118 Monoclonal Anti-MAPRE1, (N-terminal) antibody produced in mouse, clone 4F3, purified immunoglobulin, buffered aqueous solution
WH0010982M3
Monoclonal Anti-MAPRE2 antibody produced in mouse, clone 4D7, purified immunoglobulin, buffered aqueous solution