EMAIL THIS PAGE TO A FRIEND

The Journal of comparative neurology

Nectin-1 spots as a novel adhesion apparatus that tethers mitral cell lateral dendrites in a dendritic meshwork structure of the developing mouse olfactory bulb.


PMID 25967681

Abstract

Mitral cells project lateral dendrites that contact the lateral and primary dendrites of other mitral cells and granule cell dendrites in the external plexiform layer (EPL) of the olfactory bulb. These dendritic structures are critical for odor information processing, but it remains unknown how they are formed. In immunofluorescence microscopy, the immunofluorescence signal for the cell adhesion molecule nectin-1 was concentrated on mitral cell lateral dendrites in the EPL of the developing mouse olfactory bulb. In electron microscopy, the immunogold particles for nectin-1 were symmetrically localized on the plasma membranes at the contacts between mitral cell lateral dendrites, which showed bilateral darkening without dense cytoskeletal undercoats characteristic of puncta adherentia junctions. We named the contacts where the immunogold particles for nectin-1 were symmetrically accumulated "nectin-1 spots." The nectin-1 spots were 0.21 μm in length on average and the distance between the plasma membranes was 20.8 nm on average. In 3D reconstruction of serial sections, clusters of the nectin-1 spots formed a disc-like structure. In the mitral cell lateral dendrites of nectin-1-knockout mice, the immunogold particles for nectin-1 were undetectable and the plasma membrane darkening was electron-microscopically normalized, but the plasma membranes were partly separated from each other. The nectin-1 spots were further identified between mitral cell lateral and primary dendrites and between mitral cell lateral dendrites and granule cell dendritic spine necks. These results indicate that the nectin-1 spots constitute a novel adhesion apparatus that tethers mitral cell dendrites in a dendritic meshwork structure of the developing mouse olfactory bulb.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB4300621
Anti-CDH2 antibody produced in rabbit, affinity isolated antibody
SAB4500002
Anti-CDH2, C-Terminal antibody produced in rabbit, affinity isolated antibody
SAB1406354
Anti-PVRL2 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA012759
Anti-PVRL2 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2105703
Anti-PVRL2 antibody produced in rabbit, affinity isolated antibody
SAB1411165
Anti-PVRL2 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
SAB1401612
Anti-PVRL3 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA011038
Anti-PVRL3 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1401613
Anti-PVRL3 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
SAB4501099
Anti-PVRL3 antibody produced in rabbit, affinity isolated antibody
SAB2104977
Anti-PVRL3, (N-terminal) antibody produced in rabbit, affinity isolated antibody
WH0001000M1
Monoclonal Anti-CDH2 antibody produced in mouse, clone 5C8, purified immunoglobulin, buffered aqueous solution
C2542 Monoclonal Anti-N-Cadherin antibody produced in mouse, clone GC-4, ascites fluid
WH0005819M1
Monoclonal Anti-PVRL2 antibody produced in mouse, clone 2A6-2C1, purified immunoglobulin, buffered aqueous solution
SAB1402559
Monoclonal Anti-PVRL3 antibody produced in mouse, clone 1D1, purified immunoglobulin, buffered aqueous solution