EMAIL THIS PAGE TO A FRIEND

The Journal of neuroscience : the official journal of the Society for Neuroscience

Autocrine action of BDNF on dendrite development of adult-born hippocampal neurons.


PMID 26041908

Abstract

Dendrite development of newborn granule cells (GCs) in the dentate gyrus of adult hippocampus is critical for their incorporation into existing hippocampal circuits, but the cellular mechanisms regulating their dendrite development remains largely unclear. In this study, we examined the function of brain-derived neurotrophic factor (BDNF), which is expressed in adult-born GCs, in regulating their dendrite morphogenesis. Using retrovirus-mediated gene transfection, we found that deletion and overexpression of BDNF in adult-born GCs resulted in the reduction and elevation of dendrite growth, respectively. This effect was mainly due to the autocrine rather than paracrine action of BDNF, because deletion of BDNF only in the newborn GCs resulted in dendrite abnormality of these neurons to a similar extent as that observed in conditional knockout (cKO) mice with BDNF deleted in the entire forebrain. Furthermore, selective expression of BDNF in adult-born GCs in BDNF cKO mice fully restored normal dendrite development. The BDNF autocrine action was also required for the development of normal density of spines and normal percentage of spines containing the postsynaptic marker PSD-95, suggesting autocrine BDNF regulation of synaptogenesis. Furthermore, increased dendrite growth of adult-born GCs caused by voluntary exercise was abolished by BDNF deletion specifically in these neurons and elevated dendrite growth due to BDNF overexpression in these neurons was prevented by reducing neuronal activity with coexpression of inward rectifier potassium channels, consistent with activity-dependent autocrine BDNF secretion. Therefore, BDNF expressed in adult-born GCs plays a critical role in dendrite development by acting as an autocrine factor.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB1405514
Anti-BDNF antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
B9186 Anti-Brain-derived Neurotrophic Factor antibody produced in chicken, affinity isolated antibody, lyophilized powder
AV41333
Anti-DCX (AB1) antibody produced in rabbit, affinity isolated antibody
AV41332
Anti-DCX (AB2) antibody produced in rabbit, affinity isolated antibody
SAB2502091
Anti-Dcx antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB2501666
Anti-DCX antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB1405701
Anti-DCX antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
SAB2501245
Anti-DLG4 antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB1405717
Anti-DLG4 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA010122
Anti-DLG4 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1401115
Anti-DLG4 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
SAB2105773
Anti-DLG4 antibody produced in rabbit, affinity isolated antibody
D9818
Anti-Doublecortin (C-terminal) antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
D9943
Anti-Doublecortin (N-terminal) antibody produced in rabbit, ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
D9693
Anti-Doublecortin antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
SAB4300697
Anti-Doublecortin antibody produced in rabbit, affinity isolated antibody
SAB4500628
Anti-Doublecortin antibody produced in rabbit, affinity isolated antibody
SAB4500629
Anti-Doublecortin, C-Terminal antibody produced in rabbit, affinity isolated antibody
SAB1402127
Monoclonal Anti-BDNF antibody produced in mouse, clone 1B10, purified immunoglobulin, buffered aqueous solution
B5050 Monoclonal Anti-Brain-derived Neurotrophic Factor antibody produced in mouse, clone 35928.11, purified immunoglobulin, lyophilized powder
B9436 Monoclonal Anti-Brain-derived Neurotrophic Factor antibody produced in mouse, clone 37141, purified immunoglobulin, lyophilized powder
B9561 Monoclonal Anti-Brain-derived Neurotrophic Factor antibody produced in mouse, clone 35909, purified immunoglobulin, lyophilized powder
B9686 Monoclonal Anti-Brain-derived Neurotrophic Factor antibody produced in mouse, clone 37129, purified immunoglobulin, lyophilized powder
WH0001641M1
Monoclonal Anti-DCX antibody produced in mouse, clone 1G12, purified immunoglobulin, buffered aqueous solution
SAB1403727 Monoclonal Anti-DCX antibody produced in mouse, clone 3B7, purified immunoglobulin, buffered aqueous solution
SAB5200002
Monoclonal Anti-DLG4 antibody produced in mouse, clone 6G6, 1 mg/mL, purified immunoglobulin
SAB5200003
Monoclonal Anti-DLG4 antibody produced in mouse, clone 7E3, 1 mg/mL, purified immunoglobulin
P246
Monoclonal Anti-PSD95 antibody produced in mouse, clone 7E3-1B8, purified immunoglobulin, buffered aqueous solution