EMAIL THIS PAGE TO A FRIEND

Biochemical and biophysical research communications

miR-29a promotes scavenger receptor A expression by targeting QKI (quaking) during monocyte-macrophage differentiation.


PMID 26056009

Abstract

Monocyte differentiation into macrophages results in upregulation of miR-29a and scavenger receptor A (SRA) expression, while the expression of RNA binding protein, QKI is suppressed. Since SRA is a functionally important protein in atherosclerosis, it is imperative to understand the various mechanisms involved in its regulation specially the mechanism involving miR-29a. There are individual studies linking miR-29a to SRA or QKI to monocyte differentiation but there is no evidence of any linkage among them. Therefore, we intend to investigate the association among these three, if any, in terms of regulation of SRA expression. Hence, in this study, the differentiated macrophages were initially transfected with miR-29a or its inhibitor and it was shown that QKI is a direct target of mir-29a. In addition, it was also observed by bioinformatics analysis that 3'UTR in SRA mRNA has QKI binding site. So, we attempted to further understand the role of QKI in SRA regulation. The macrophages were manipulated either with overexpression of QKI or by its ablation and it was observed that QKI suppressed SRA at the transcriptional level. Moreover, with the help of luciferase reporter vector, it was shown that QKI inhibited SRA transcription by binding to QRE region in its 3'UTR mRNA. Furthermore, to link the QKI mediated regulation of SRA expression with its functional activity; we analyzed lipid uptake capacity of macrophages transfected with either ectopic OKI plasmid or ablated for QKI. It was observed that, indeed, QKI upregulation inhibits lipid uptake by repressing SRA expression. Overall, our study demonstrates that miR-29a inhibits QKI, which in turn results in upregulation of SRA and lipid uptake.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB1406969
Anti-APBB3 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA005571
Anti-APBB3 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2105846
Anti-APBB3, (N-terminal) antibody produced in rabbit, affinity isolated antibody
SAB1303264
ANTI-EIF2C2 (N-TERM) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
HPA000272
Anti-MSR1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA019123
Anti-QKI antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2701257
Anti-QKI antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB1302639
ANTI-SRA1 (C-TERM) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
HPA044598 Anti-SRA1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA050153
Anti-SRA1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2107646
Anti-SRA1 antibody produced in rabbit, affinity isolated antibody
SAB2105344
Anti-SRA1, (N-terminal) antibody produced in rabbit, affinity isolated antibody
SAB4200085
Monoclonal Anti-AGO2 antibody produced in rat, ~1.5 mg/mL, clone 11A9, purified immunoglobulin
WH0027161M1
Monoclonal Anti-EIF2C2 antibody produced in mouse, clone 2E12-1C9, purified immunoglobulin, buffered aqueous solution
SAB1405034
Monoclonal Anti-EIF2C2 antibody produced in mouse, clone 4D1-1D1, purified immunoglobulin, buffered aqueous solution
WH0004481M1
Monoclonal Anti-MSR1 antibody produced in mouse, clone 2G8, purified immunoglobulin, buffered aqueous solution
SAB1404085
Monoclonal Anti-MSR1 antibody produced in mouse, clone 2G9, purified immunoglobulin, buffered aqueous solution
SAB5300015
Monoclonal Anti-SRA1 antibody produced in mouse, clone 1D4H8, ascites fluid
SAB5300016
Monoclonal Anti-SRA1 antibody produced in mouse, clone 7H1G1, ascites fluid