EMAIL THIS PAGE TO A FRIEND

Analytical and bioanalytical chemistry

Critical evaluation of indirect methods for the determination of deoxynivalenol and its conjugated forms in cereals.


PMID 26065425

Abstract

A critical assessment of three previously published indirect methods based on acidic hydrolysis using superacids for the determination of "free" and "total" deoxynivalenol (DON) was carried out. The modified mycotoxins DON-3-glucoside (D3G), 3-acetyl-DON (3ADON), and 15-acetyl-DON (15ADON) were chosen as model analytes. The initial experiments focused on the stability/degradation of DON under hydrolytic conditions and the ability to release DON from the modified forms. Acidic conditions that were capable of cleaving D3G, 3ADON, and 15ADON to DON were not found, raising doubts over the efficacy of previously published indirect methods for total DON determination. Validation of these indirect methods for wheat, maize, and barley using UHPLC-MS/MS was performed in order to test the accuracy of the generated results. Validation data for DON, D3G, 3ADON, and 15ADON in nonhydrolyzed and hydrolyzed matrices were obtained. Under the tested conditions, DON was not released from D3G, 3ADON, or 15ADON after hydrolysis and thus none of the published methods were able to cleave the modified forms of DON. In addition to acids, alkaline hydrolysis with KOH for an extended time and at elevated temperatures was also tested. 3ADON and 15ADON were cleaved under the alkaline pH caused by the addition of KOH or aqueous K2CO3 to "neutralize" the acidic sample extracts in the published studies. The published additional DON increase after hydrolysis may have been caused by huge differences in matrix effects and the recovery of DON in nonhydrolyzed and hydrolyzed matrices as well as by the alkaline cleavage of 3ADON or 15ADON after the neutralization of hydrolyzed extracts.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

220116
2-Hydroxybutyric acid sodium salt, 97%
C4H7NaO3
A1542
Ammonium acetate, for molecular biology, ≥98%
C2H7NO2
A7330
Ammonium acetate, BioXtra, ≥98%
C2H7NO2
372331
Ammonium acetate, 99.999% trace metals basis
C2H7NO2
A7262
Ammonium acetate, reagent grade, ≥98%
C2H7NO2
A2706
Ammonium acetate solution, for molecular biology, 7.5 M
C2H7NO2
B6768
Boric acid, BioReagent, for molecular biology, suitable for cell culture, suitable for plant cell culture, ≥99.5%
H3BO3
B7901
Boric acid, for electrophoresis, ≥99.5%
H3BO3
B2645
Boric acid, tablet, 1 g boric acid per tablet
H3BO3
B9645
Boric acid, cell culture tested, plant cell culture tested, ≥99.5%
H3BO3
B1934
Boric acid, Biotechnology Performance Certified, ≥99.5% (titration), Cell Culture Tested
H3BO3
202878
Boric acid, 99.999% trace metals basis
H3BO3
339067
Boric acid, 99.97% trace metals basis
H3BO3
B7660
Boric acid, BioXtra, ≥99.5%
H3BO3
15663
Boric acid, BioUltra, for molecular biology, ≥99.5% (T)
H3BO3
426148
Boric acid-11B, ≥99 atom % 11B
H311BO3
791725
Citric acid, anhydrous, free-flowing, Redi-Dri, ACS reagent, ≥99.5%
C6H8O7
C2404
Citric acid, anhydrous, cell culture tested, plant cell culture tested
C6H8O7
251275
Citric acid, ACS reagent, ≥99.5%
C6H8O7
C83155
Citric acid, 99%
C6H8O7
C0759
Citric acid, 99%
C6H8O7
27487
Citric acid, BioUltra, anhydrous, ≥99.5% (T)
C6H8O7
W230618
Citric acid, ≥99.5%, FCC, FG
C6H8O7
561932
Deblock trichloroacetic acid solution, 3 % (w/v) in methylene chloride
C2HCl3O2
D0156
Deoxynivalenol
C15H20O6
P1472
Potassium carbonate, meets USP testing specifications
CK2O3
367877
Potassium carbonate, 99.995% trace metals basis
CK2O3
590681
Potassium carbonate, anhydrous, powder, 99.99% trace metals basis
CK2O3
60108
Potassium carbonate, BioUltra, anhydrous, ≥99.0% (T)
CK2O3
757551
Potassium hydroxide, anhydrous, ≥99.97% trace metals basis
HKO