EMAIL THIS PAGE TO A FRIEND

ACS nano

Sizing Individual Au Nanoparticles in Solution with Sub-Nanometer Resolution.


PMID 26083098

Abstract

Resistive-pulse sensing has generated considerable interest as a technique for characterizing nanoparticle suspensions. The size, charge, and shape of individual particles can be estimated from features of the resistive pulse, but the technique suffers from an inherent variability due to the stochastic nature of particles translocating through a small orifice or channel. Here, we report a method, and associated automated instrumentation, that allows repeated pressure-driven translocation of individual particles back and forth across the orifice of a conical nanopore, greatly reducing uncertainty in particle size that results from streamline path distributions, particle diffusion, particle asphericity, and electronic noise. We demonstrate ∼0.3 nm resolution in measuring the size of nominally 30 and 60 nm radius Au nanoparticles of spherical geometry; Au nanoparticles in solution that differ by ∼1 nm in radius are readily distinguished. The repetitive translocation method also allows differentiating particles based on surface charge density, and provides insights into factors that determine the distribution of measured particle sizes.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

S7907
Sodium phosphate dibasic, BioXtra, ≥99.0%
HNa2O4P
S3264
Sodium phosphate dibasic, for molecular biology, ≥98.5% (titration)
HNa2O4P
S5136
Sodium phosphate dibasic, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%
HNa2O4P
255793
Sodium phosphate dibasic, 99.95% trace metals basis
HNa2O4P
S0876
Sodium phosphate dibasic, ReagentPlus®, ≥99.0%
HNa2O4P
71636
Sodium phosphate dibasic, BioUltra, for molecular biology, ≥99.5% (T)
HNa2O4P
W239901
Sodium phosphate dibasic, 99%, FG
HNa2O4P
94046
Sodium phosphate dibasic solution, BioUltra, 0.5 M in H2O
HNa2O4P
RDD007
Sodium phosphate monobasic, anhydrous, free-flowing, Redi-Dri, ≥99.0%
H2NaO4P
S8282
Sodium phosphate monobasic, BioXtra, ≥99.0%
H2NaO4P
S3139
Sodium phosphate monobasic, BioReagent, for molecular biology, anhydrous, ≥98%
H2NaO4P
S5011
Sodium phosphate monobasic, BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0% (titration)
H2NaO4P
S2554
Sodium phosphate monobasic, meets USP testing specifications, anhydrous
H2NaO4P
S6566
Sodium phosphate monobasic, Biotechnology Performance Certified, Cell Culture Tested
H2NaO4P
229903
Sodium phosphate monobasic, 99.999% trace metals basis
H2NaO4P
S0751
Sodium phosphate monobasic, ReagentPlus®, ≥99.0%
H2NaO4P
74092
Sodium phosphate monobasic solution, BioUltra, 5 M in H2O
H2NaO4P
609773
Sodium phosphate monobasic-16O4, 99.9 atom % 16O
H2Na16O4P