EMAIL THIS PAGE TO A FRIEND

Monoclonal antibodies in immunodiagnosis and immunotherapy

Characterization of Monoclonal Antibody LpMab-7 Recognizing Non-PLAG Domain of Podoplanin.


PMID 26090595

Abstract

Podoplanin (PDPN/Aggrus/T1α), a platelet aggregation-inducing type I transmembrane sialoglycoprotein, is involved in tumor invasion and metastasis. Furthermore, podoplanin expression was reported to be involved in poor prognosis of several cancers. Although many anti-podoplanin monoclonal antibodies (MAbs), such as NZ-1 and D2-40, have been established, those epitopes are limited to platelet aggregation-stimulating (PLAG) domain of podoplanin. In this study, we developed and characterized a novel anti-podoplanin MAb, LpMab-7, that is more sensitive than NZ-1 in immunohistochemistry. We identified the minimum epitope of LpMab-7 as Arg79-Leu83 of human podoplanin, which is different from PLAG domain, using ELISA, Western blot analysis, and flow cytometry. Because LpMab-7 has high sensitivity against podoplanin, LpMab-7 is expected to be useful for molecular targeting therapy for podoplanin-expressing cancers.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

A9917 Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat, affinity isolated antibody, buffered aqueous solution
A2304 Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat, affinity isolated antibody, buffered aqueous solution
A3682 Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB3701029 Anti-Mouse IgG (Fc specific), F(ab′)2 fragment, highly cross adsorbed-Peroxidase antibody produced in goat, affinity isolated antibody, lyophilized powder
A0168 Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat, affinity isolated antibody
A2554 Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat, affinity isolated antibody, buffered aqueous solution
A9309 Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB3701023 Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in rabbit, affinity isolated antibody, lyophilized powder
A3673 Anti-Mouse IgG (γ-chain specific)−Peroxidase antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB3701037 Anti-Mouse IgG (γ-chain specific)-Peroxidase antibody produced in rabbit, affinity isolated antibody, lyophilized powder
SAB3701131 Anti-Mouse IgG (H+L), F(ab′)2 fragment-Peroxidase antibody produced in goat, affinity isolated antibody, lyophilized powder
SAB3701159 Anti-Mouse IgG (H+L), F(ab) fragment-Peroxidase antibody produced in goat, affinity isolated antibody, lyophilized powder
SAB3701165 Anti-Mouse IgG (H+L), F(ab) fragment-Peroxidase antibody produced in rabbit, affinity isolated antibody, lyophilized powder
SAB3701116 Anti-Mouse IgG (H+L)-Peroxidase antibody produced in chicken, affinity isolated antibody, lyophilized powder
SAB3701105
Anti-Mouse IgG (H+L)-Peroxidase antibody produced in donkey, affinity isolated antibody, lyophilized powder
SAB3701083
Anti-Mouse IgG (H+L)-Peroxidase antibody produced in rabbit, affinity isolated antibody, lyophilized powder
SAB3701095
Anti-Mouse IgG (H+L)-Peroxidase antibody produced in sheep, affinity isolated antibody, lyophilized powder
A4416 Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat, affinity isolated antibody, buffered aqueous solution
A8924 Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat, affinity isolated antibody, buffered aqueous solution
A5278 Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat, IgG fraction of antiserum, buffered aqueous solution
A9044 Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
A5906 Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in sheep, affinity isolated antibody, buffered aqueous solution
A6782 Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in sheep, affinity isolated antibody, buffered aqueous solution
SAB3701015 Anti-Mouse IgG F(ab′)2, F(ab′)2 fragment, highly cross absorbed-Peroxidase antibody produced in goat, affinity isolated antibody, lyophilized powder
SAB3701004 Anti-Mouse IgG F(ab′)2, highly cross adsorbed-Peroxidase antibody produced in rabbit, affinity isolated antibody, lyophilized powder
SAB3700993 Anti-Mouse IgG F(ab′)2-Peroxidase antibody produced in goat, affinity isolated antibody, lyophilized powder
SAB3701003 Anti-Mouse IgG F(ab′)2-Peroxidase antibody produced in rabbit, affinity isolated antibody, lyophilized powder
A7502
DL-Alanine, ≥99% (HPLC)
C3H7NO2
W381810
DL-Alanine, ≥99%, FCC, FG
C3H7NO2
H9627
Hematoxylin
C16H14O6 · xH2O