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Taiwanese journal of obstetrics & gynecology

The role of nitric oxide in the outgrowth of trophoblast cells on human umbilical vein endothelial cells.


PMID 26166331

Abstract

Embryo implantation is a complex process that requires coordinated trophoblast-endometrial interactions. Previous studies demonstrated that the identification of nitric oxide synthase (NOS) in trophoblast cells and the remodeling of the implantation process by nitric oxide (NO) support the important role of NO during implantation. However, the role of NO in trophoblast-endometrial interactions is unclear and is therefore examined in this study. We cocultured BeWo trophoblast spheroids with human umbilical vein endothelial cell (HUVEC) monolayers to mimic the trophoblast-endometrial interaction. N(ω)-Nitro-l-arginine methyl ester hydrochloride (l-NAME), a competitive inhibitor of NOS, and sodium nitroprusside (SNP), an NO donor, were used to test the role of NO in the trophoblast-endometrial interaction. l-NAME diminished spheroid expansion on HUVEC monolayers in a concentration-dependent manner (pxa0xa00.05). Significant suppression of spheroid expansion was found at the higher dose (1mM) of SNP (pxa0