EMAIL THIS PAGE TO A FRIEND

Nature communications

Arginylation regulates purine nucleotide biosynthesis by enhancing the activity of phosphoribosyl pyrophosphate synthase.


PMID 26175007

Abstract

Protein arginylation is an emerging post-translational modification that targets a number of metabolic enzymes; however, the mechanisms and downstream effects of this modification are unknown. Here we show that lack of arginylation renders cells vulnerable to purine nucleotide synthesis inhibitors and affects the related glycine and serine biosynthesis pathways. We show that the purine nucleotide biosynthesis enzyme PRPS2 is selectively arginylated, unlike its close homologue PRPS1, and that arginylation of PRPS2 directly facilitates its biological activity. Moreover, selective arginylation of PRPS2 but not PRPS1 is regulated through a coding sequence-dependent mechanism that combines elements of mRNA secondary structure with lysine residues encoded near the N-terminus of PRPS1. This mechanism promotes arginylation-specific degradation of PRPS1 and selective retention of arginylated PRPS2 in vivo. We therefore demonstrate that arginylation affects both the activity and stability of a major metabolic enzyme.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

AV40173
Anti-ACTB antibody produced in rabbit, affinity isolated antibody, lyophilized powder
SAB2100037
Anti-ACTB antibody produced in rabbit, affinity isolated antibody
GW23014 Anti-Actin, Cytoplasmic 1 antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
SAB3500350
Anti-β-Actin antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
SAB2700018
Anti-PRPS1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB2700019
Anti-PRPS1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
HPA043760
Anti-PRPS1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2700452
Anti-PRPS1L1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
A1895
Aurintricarboxylic acid, practical grade, ≥85% (titration), powder
C22H14O9
A0885
Aurintricarboxylic acid ammonium salt, powder
C22H23N3O9
A36883
Aurintricarboxylic acid ammonium salt, ACS reagent
C22H23N3O9
WH0000060M1
Monoclonal Anti-ACTB antibody produced in mouse, clone 3G4-F9, purified immunoglobulin, buffered aqueous solution
SAB1403520
Monoclonal Anti-ACTB antibody produced in mouse, clone 1E7, purified immunoglobulin, buffered aqueous solution
WH0221823M1
Monoclonal Anti-PRPS1L1 antibody produced in mouse, clone 5E10, purified immunoglobulin, buffered aqueous solution
270970
Pyridine, anhydrous, 99.8%
C5H5N
W296600
Pyridine, ≥99%
C5H5N
24-6510
Pyridine, SAJ first grade, ≥99.0%
C5H5N
190780
Sodium trichloroacetate, 97%
C2Cl3NaO2