EMAIL THIS PAGE TO A FRIEND

Journal of inflammation (London, England)

A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers.


PMID 26213499

Abstract

Serum and plasma calprotectin concentration is shown to be elevated when neutrophils are activated, and may therefore be used as a marker for inflammatory diseases. A serum calprotectin immunoassay was developed based on calprotectin values observed in samples from the intensive care unit. The polyclonal avian antibodies were raised and affinity purified with calprotectin antigens. The performance was tested and it was observed that the assay was linear in the range 0.3-24.7xa0mg/L, the limit of quantitation was observed to be lower than 0.3xa0mg/L, no antigen excess was observed up to 54xa0mg/L, all CVs were lower than 1.8xa0% in the precision study, the calibration curve stability was longer than 6xa0weeks, and there was no significant interference detected for haemoglobin, intralipid or bilirubin. The serum calprotectin immunoassay presented in this paper performs well within the criteria carefully set from the limited clinical experience obtained in both serum and plasma. In addition it is commutable with Bühlmann MRP8/14 ELISA.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

15270
Biuret
C2H5N3O2
B54207
Biuret, 97%
C2H5N3O2