EMAIL THIS PAGE TO A FRIEND

The Journal of biological chemistry

ETS-related transcription factors ETV4 and ETV5 are involved in proliferation and induction of differentiation-associated genes in embryonic stem (ES) cells.


PMID 26224636

Abstract

The pluripotency and self-renewal capacity of embryonic stem (ES) cells is regulated by several transcription factors. Here, we show that the ETS-related transcription factors Etv4 and Etv5 (Etv4/5) are specifically expressed in undifferentiated ES cells, and suppression of Oct3/4 results in down-regulation of Etv4/5. Simultaneous deletion of Etv4 and Etv5 (Etv4/5 double knock-out (dKO)) in ES cells resulted in a flat, epithelial cell-like appearance, whereas the morphology changed into compact colonies in a 2i medium (containing two inhibitors for GSK3 and MEK/ERK). Expression levels of self-renewal marker genes, including Oct3/4 and Nanog, were similar between wild-type and dKO ES cells, whereas proliferation of Etv4/5 dKO ES cells was decreased with overexpression of cyclin-dependent kinase inhibitors (p16/p19, p15, and p57). A differentiation assay revealed that the embryoid bodies derived from Etv4/5 dKO ES cells were smaller than the control, and expression of ectoderm marker genes, including Fgf5, Sox1, and Pax3, was not induced in dKO-derived embryoid bodies. Microarray analysis demonstrated that stem cell-related genes, including Tcf15, Gbx2, Lrh1, Zic3, and Baf60c, were significantly repressed in Etv4/5 dKO ES cells. The artificial expression of Etv4 and/or Etv5 in Etv4/5 dKO ES cells induced re-expression of Tcf15 and Gbx2. These results indicate that Etv4 and Etv5, potentially through regulation of Gbx2 and Tcf15, are involved in the ES cell proliferation and induction of differentiation-associated genes in ES cells.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

AV100591
Anti-NANOG (AB1) antibody produced in rabbit, affinity isolated antibody
AV47988
Anti-NANOG (AB2) antibody produced in rabbit, affinity isolated antibody
SAB2500670
Anti-NANOG antibody produced in goat, affinity isolated antibody, buffered aqueous solution
N4413 Anti-Nanog antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
SAB4300680
Anti-Nanog antibody produced in rabbit, affinity isolated antibody
SAB3500389
Anti-NANOG antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB2104002
Anti-NANOG antibody produced in rabbit, affinity isolated antibody
SAB2104004
Anti-NANOG, (N-terminal) antibody produced in rabbit, affinity isolated antibody
SAB2500671
Anti-NANOG, C-Terminal antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB1408519
Anti-NANOGP8 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
SAB4501671
Anti-NANOGP8 antibody produced in rabbit, affinity isolated antibody
SAB4502259
Anti-OCT3 antibody produced in rabbit, affinity isolated antibody
SAB2501454
Anti-POU5F1 antibody produced in goat, affinity isolated antibody, buffered aqueous solution
AV100956
Anti-POU5F1 antibody produced in rabbit, IgG fraction of antiserum
SAB2101850
Anti-POU5F1 antibody produced in rabbit, affinity isolated antibody
SAB3500316
Anti-POU5F1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB2701958
Anti-POU5F1 antibody produced in rabbit
SAB2701960
Anti-POU5F1 antibody produced in rabbit
SAB2701972
Anti-POU5F1 antibody produced in rabbit
AV44026
Anti-SLC22A3 antibody produced in rabbit, affinity isolated antibody
HPA029750
Anti-SLC22A3 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB4502815
Anti-SLC22A3 antibody produced in rabbit, affinity isolated antibody
SAB2106660
Anti-SLC22A3 antibody produced in rabbit, affinity isolated antibody
SAB1406815
Anti-SLC22A8 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA044174
Anti-SLC22A8 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
N3038
Monoclonal Anti-Nanog antibody produced in mouse, ~2 mg/mL, clone NNG-811, purified immunoglobulin, buffered aqueous solution
WH0079923M1
Monoclonal Anti-NANOG antibody produced in mouse, clone 2C11, purified immunoglobulin, buffered aqueous solution
SAB1405280
Monoclonal Anti-NANOG antibody produced in mouse, clone 2E11, purified immunoglobulin, buffered aqueous solution
WH0005460M1
Monoclonal Anti-POU5F1 antibody produced in mouse, clone 1D2, purified immunoglobulin, buffered aqueous solution
SAB1404228
Monoclonal Anti-POU5F1, (C-terminal) antibody produced in mouse, clone 4F8, purified immunoglobulin, buffered aqueous solution