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Cancer cell international

Antitumoral potency of methanolic extract from Nitraria retusa leaves via its immunomodulatory effect.


PMID 26309426

Abstract

The purpose of this study was to assess the antitumoral effect of the methanol extract (MeOH) from Nitraria retusa leaves and to investigate its immunomodulatory activity that mediated the prevention of tumor progression in tumor-bearing mice. Balb/c mice weighing 18-20xa0g were subcutaneously implanted with B16-F10 cells then injected intra-peritoneally, 7xa0days later with (200xa0mg/kg bw) of MeOH extract, for 21xa0days. After euthanization on day 21, the tumors were weighed. Lymphocyte proliferation, cytotoxic T lymphocyte (CTL) and NK activity were evaluated using the MTT assay. Macrophage phagocytosis was studied by measuring their lysosomal activity and nitric oxide production. The methanol extract inhibited significantly the growth of the implanted tumor, and increased remarkably splenocyte proliferation as well as NK and CTL activities, in tumor-bearing mice. It also promoted lysosomal activity of treated animal macrophages. Our findings suggest that antitumoral effect of MeOH extract is related with to immunomodulatory activity.