EMAIL THIS PAGE TO A FRIEND

Scientific reports

Increased expression of surface CD44 in hypoxia-DCs skews helper T cells toward a Th2 polarization.


PMID 26323509

Abstract

A low partial oxygen pressure (hypoxia) occurs in many pathological environments, such as solid tumors and inflammatory lesions. Understanding the cellular response to hypoxic stress has broad implications for human diseases. As we previously reported, hypoxia significantly altered dendritic cells (DCs) to a DC2 phenotype and promoted a Th2 polarization of naïve T cells with increased IL-4 production. However, the underlying mechanisms still remain largely unknown. In this study, we found the over-expression of surface CD44 in DCs was involved in this process via ligand binding. Further investigation showed hypoxia could reduce the surface expression of membrane type 1 metalloprotease (MT1-MMP) via down-regulating the kinesin-like protein KIF2A, which subsequently alleviated the shedding of CD44 from DCs. Moreover, KIF2A expression was found negatively regulated by HIF-1α in hypoxic microenvironment. These results suggest a previously uncharacterized mechanism by which hypoxia regulates the function of DCs via KIF2A/MT1-MMP/CD44 axis, providing critical information to understand the immune response under hypoxia.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

GW22783 Anti-CD14 antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
SAB2500215
Anti-CD14 antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB1405579
Anti-CD14 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA001887
Anti-CD14 antibody produced in rabbit, Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA002127
Anti-CD14 antibody produced in rabbit, Ab3, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2101261
Anti-KIF2A (ab1) antibody produced in rabbit, affinity isolated antibody
SAB2101262
Anti-KIF2A (ab2) antibody produced in rabbit, affinity isolated antibody
HPA004716
Anti-KIF2A antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
AV34033
Anti-KIF2A antibody produced in rabbit, affinity isolated antibody
SAB4501901
Anti-MMP-14, C-Terminal antibody produced in rabbit, affinity isolated antibody
SAB4501902
Anti-MMP14 (Cleaved-Tyr112) antibody produced in rabbit, affinity isolated antibody
GW21125 Anti-MMP14 antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
SAB1411900
ANTI-MT1A antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA041152
Anti-MT1E antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB1306983
ANTI-MTNR1A (CENTER) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB2501240
Anti-MTNR1A antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB2700731 Anti-MTNR1A antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
AMAB90897
Monoclonal Anti-CD14 antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL1637, purified immunoglobulin, buffered aqueous glycerol solution
AMAB90898
Monoclonal Anti-CD14 antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL1638, purified immunoglobulin, buffered aqueous glycerol solution
C7673
Monoclonal Anti-CD14 antibody produced in mouse, clone UCHM-1, purified immunoglobulin, buffered aqueous solution
SAB4700097
Monoclonal Anti-CD14 antibody produced in mouse, clone MEM-15, purified immunoglobulin, buffered aqueous solution
SAB4700103 Monoclonal Anti-CD14 antibody produced in mouse, clone MEM-18, purified immunoglobulin, buffered aqueous solution
SAB4700669 Monoclonal Anti-CD14 antibody produced in mouse, clone B-A8, purified immunoglobulin, buffered aqueous solution
WH0003796M1
Monoclonal Anti-KIF2 antibody produced in mouse, clone 2D4, purified immunoglobulin, buffered aqueous solution
79346
Phorbol 12-myristate 13-acetate, synthetic, ≥98.0% (TLC)
C36H56O8
P8139
Phorbol 12-myristate 13-acetate, ≥99% (TLC), film or powder
C36H56O8