EMAIL THIS PAGE TO A FRIEND

Journal of immunology research

Multifunctional Analysis of CD4+ T-Cell Response as Immune-Based Model for Tuberculosis Detection.


PMID 26339657

Abstract

Mono- and multifunctional specific CD4(+) and CD8(+) T-cell responses were evaluated to improve the immune-based detection of active tuberculosis (TB) and latent infection (LTBI). We applied flow cytometry to investigate cytokines profile (IFN-γ, TNF-α, and IL-2) of T cells after stimulation with TB antigens in 28 TB-infected subjects (18 active TB and 10 LTBI) and 10 uninfected controls. Cytokines production by CD4(+) T cells at single-cell levels was higher in TB-infected subjects than uninfected controls (P < 0.0001). Assigning to activated CD4(+) T cells, producing any of the three cytokines, a cut-off >0.45%, it was possible to differentiate TB-infected (>0.45%) by uninfected subjects (<0.45%). Among TB-infected subjects, the frequencies of multifunctional CD4(+) T cells, simultaneously producing all 3 cytokines, are lower in active TB than LTBI subjects (P = 0.003). Thus, assigning to triple-positive CD4(+) T cells a cut-off <0.182%, TB-infected individuals could be classified as active TB subjects (<0.182%) or LTBI subjects (>0.182%). The magnitude of CD8(+) T-cell responses showed no differences between active TB and LTBI. Multifunctional CD4(+) T-cell responses could have the potential to identify at single time point subjects without TB infection and patients having active or latent TB.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB2501381
Anti-Cd28 antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB2501674
Anti-CD28 antibody produced in goat, affinity isolated antibody, buffered aqueous solution
SAB1405583
Anti-CD28 antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
C7831 Anti-CD28 antibody, Mouse monoclonal, clone CD28.2, purified from hybridoma cell culture
HPA004252
Anti-CD4 antibody produced in rabbit, Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
HPA004472
Anti-CD4 antibody produced in rabbit, Ab2, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB4503583
Anti-CD4, C-Terminal antibody produced in rabbit, affinity isolated antibody
SAB1405577
Anti-CD8A antibody produced in mouse, purified immunoglobulin, buffered aqueous solution
HPA037756 Anti-CD8A antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
GW22461F Anti-Interleukin-2 antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
I8398 Anti-Interleukin-2 antibody produced in goat, IgG fraction of antiserum, lyophilized powder
I8273 Anti-Interleukin-2 antibody produced in goat, IgG fraction of antiserum, lyophilized powder
I7401 Anti-Interleukin-2 antibody produced in goat, affinity isolated antibody, lyophilized powder
I7784 Anti-Interleukin-2 antibody produced in goat, affinity isolated antibody, lyophilized powder
A0781
Aphidicolin from Nigrospora sphaerica, ≥98% (HPLC), powder
C20H34O4
SAB4700135 Monoclonal Anti-CD28 antibody produced in mouse, low endotoxin, clone CD28.2, purified immunoglobulin, buffered aqueous solution
SAB4700739 Monoclonal Anti-CD28 antibody produced in mouse, clone JJ319, purified immunoglobulin, buffered aqueous solution
SAB4700751
Monoclonal Anti-CD28 antibody produced in mouse, clone CD28.2, purified immunoglobulin, buffered aqueous solution
SAB4700059
Monoclonal Anti-CD4 , (N-terminal) antibody produced in mouse, clone MEM-241, purified immunoglobulin, buffered aqueous solution
C1805 Monoclonal Anti-CD4 antibody produced in mouse, clone Q4120, purified immunoglobulin, buffered aqueous solution
SAB4700054 Monoclonal Anti-CD4 antibody produced in mouse, clone MEM-16, purified immunoglobulin, buffered aqueous solution
SAB4700065
Monoclonal Anti-CD4 antibody produced in mouse, clone MEM-115, purified immunoglobulin, buffered aqueous solution
SAB4700733 Monoclonal Anti-CD4 antibody produced in mouse, clone OX-35, purified immunoglobulin, buffered aqueous solution
SAB4100489
Monoclonal Anti-CD4 antibody produced in mouse, clone CD4 -A409, culture supernatant
AMAB90754
Monoclonal Anti-CD4 antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL0395, purified immunoglobulin, buffered aqueous glycerol solution
C7423
Monoclonal Anti-CD8 antibody produced in mouse, clone UCHT-4, purified immunoglobulin, buffered aqueous solution
SAB4700084 Monoclonal Anti-CD8 antibody produced in mouse, clone MEM-31, purified immunoglobulin, buffered aqueous solution
SAB4700089 Monoclonal Anti-CD8 antibody produced in mouse, clone MEM-87, purified immunoglobulin, buffered aqueous solution
SAB1402142
Monoclonal Anti-CD8A antibody produced in mouse, clone 4B9, purified immunoglobulin, buffered aqueous solution
WH0003558M3 Monoclonal Anti-IL2 antibody produced in mouse, clone 3C8, purified immunoglobulin, buffered aqueous solution