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Theriogenology

Effect of estrogen on the expression of GnRH and kisspeptin in the hypothalamus of rats during puberty.


PMID 26365586

Abstract

The aim of this study was to assess whether changes in kisspeptin and GnRH levels could be attributed to sex steroids at puberty onset. We used the ovariectomy (OVX) model in rats treated with 17β-estradiol (E2; OVXxa0+xa0E2), or oil (OVXxa0+xa0oil), and in intact rats treated with E2 (intactxa0+xa0E2) or oil only (intactxa0+xa0oil) to determine gene expression changes of Kiss1 and Gnrh1 in the hypothalamus and protein expression of kisspeptin and GnRH in the different areas of the hypothalamus. In the intactxa0+xa0E2 and OVXxa0+xa0E2 rats on the day of thexa0onset of puberty, GnRH-immunoreactive (ir) cell numbers decreased (Pxa0xa00.05) was found in the paraventricularis nucleus for GnRH-ir or kisspeptin-ir cells. Additionally, levels of Kiss1 and Gnrh1 messenger RNA in the hypothalamus were significantly higher (Pxa0