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Cell biology international

Mitochondrial apoptosis and autophagy in the process of adipose-derived stromal cell differentiation into astrocytes.


PMID 26399453

Abstract

This study aimed to investigate the phenomenon of mitochondrial apoptosis and autophagy in the process of adipose-derived stromal cell (ADSC) differentiation into astrocytes for 48 h, 7 days, 14 days, and 21 days. Immunocytochemistry and Western blotting indicated that the expression of GFAP reached a peak on the 7th day (P < 0.05). The expression of Bcl-2 was decreased (P < 0.05), but Bax, Cyt-c, and LC3 increased with time and reached a maximum on the 14th day (P < 0.05). TEM was used to observe the ultrastructure of mitochondria, apoptosis, and autophagy. An MTT assay indicated that the number of surviving cells decreased. During ADSC differentiation into astrocytes, the effect of Bcl-2 inhibition on apoptosis decreased, whereas mitochondrial apoptosis and autophagy were enhanced; however, this selective autophagy could not eliminate all the damaged mitochondria, leading to mitochondrial apoptosis.