EMAIL THIS PAGE TO A FRIEND

Cell biochemistry and function

Heat shock protein 20 (HSP20) is a novel substrate for protein kinase D1 (PKD1).


PMID 26443497

Abstract

Heat shock protein 20 (HSP20) has cardioprotective qualities, which are triggered by PKA phosphorylation. PKD1 is also a binding partner for HSP20, and this prompted us to investigate whether the chaperone was a substrate for PKD1. We delineate the PKD1 binding sites on HSP20 and show for the first time HSP20 is a substrate for PKD1. Phosphorylation of HSP20 by PKD1 is diminished by pharmacological or siRNA reduction of PKD1 activity and is enhanced following PKD1 activation. Our results suggest that both PKA and PKD1 can both phosphorylate HSP20 on serine 16 but that PKA is the most dominant.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB2501673
Anti-GNB2L1 antibody produced in goat, affinity isolated antibody, buffered aqueous solution
AV40625
Anti-GNB2L1 antibody produced in rabbit, affinity isolated antibody
SAB2107624
Anti-GNB2L1 antibody produced in rabbit, affinity isolated antibody
SAB2103410
Anti-GNB2L1, (N-terminal) antibody produced in rabbit, affinity isolated antibody
SAB4300060
Anti-phospho-PRKD1 (pSer738) antibody produced in rabbit, affinity isolated antibody
SAB4300075
Anti-phospho-PRKD1 (pSer910) antibody produced in rabbit, affinity isolated antibody
SAB4502371
Anti-PKD1 antibody produced in rabbit, affinity isolated antibody
SAB4502372
Anti-PKD1 antibody produced in rabbit, affinity isolated antibody
SAB4501023
Anti-PKD1, C-Terminal antibody produced in rabbit, affinity isolated antibody
SAB4502370
Anti-PKD1, C-Terminal antibody produced in rabbit, affinity isolated antibody
K4767
Anti-PRKD1 (376-390) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
SAB4300399
Anti-PRKD1 (Ab-738) antibody produced in rabbit, affinity isolated antibody
SAB4300400
Anti-PRKD1 (Ab-910) antibody produced in rabbit, affinity isolated antibody
HPA029834
Anti-PRKD1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
SAB2107622
Anti-PRKD1 antibody produced in rabbit, affinity isolated antibody
P3987 Anti-Protein Kinase D antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
R2030
Anti-RACK1 (C-terminal) antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution, immunoprecipitation: 5 μg
R1905
Anti-RACK1 (N-terminal) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
C1749
Monoclonal Anti-Polycystin-1 antibody produced in mouse, clone PKD46, tissue culture supernatant, buffered aqueous solution