EMAIL THIS PAGE TO A FRIEND

Molecular medicine reports

Epigenetic modifications of histone H3 during the transdifferentiation of Thy-1(+) Lin(‑) bone marrow cells into hepatocytes.


PMID 26459745

Abstract

The epigenetic modifications during the transdifferentiation of adult stem cells remain to be fully elucidated. In the present study, the histone H3 modifications during the transdifferentiation of rat Thy‑1(+) Lin(‑) bone marrow cells into hepatocytes in vitro were examined, which involved performing hepatocyte growth factor-mediated transdifferentiation of bone marrow Thy-1(+) Lin(‑) cells into hepatic lineage cells. Subsequently, the hepatocyte-specific markers, cytokeratin‑18 (CK‑18), albumin (ALB) and α‑fetoprotein (AFP) were examined by immunofluorescence staining or reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Changes in the key pluripotency factor, octamer‑binding transcription factor 4 (OCT4) and histone modifications, including the dimethylation and acetylation of H3 at lysine 9 (H3K9me2 and H3K9ac), lysine 14 (H3K14me2 and H3K14ac) and lysine 27 (H3K27me2 and H3K27ac), were also investigated by RT-qPCR, immunofluorescence staining or western blot analysis The mRNA expression levels of AFP and ALB were detected in the bone marrow stem cell‑derived hepatic lineage cells on days 7 and 14 following induction, and CK‑18 was detected on day 14 following induction. During the transdifferentiation of the bone marrow Thy‑1(+) Lin(‑) cells into hepatocytes, the mRNA expression of OCT4 was significantly reduced, and the levels of H3K9me2, H3K9ac, H3K14me2, H3K14ac, H3K27me2 and H3K27ac were increased significantly, compared with the levels at baseline (P<0.05). Therefore, the results of the present study demonstrated that histone H3 modifications at lysine 9, 14 and 27 are involved in the regulation of transcription during the transdifferentiation of bone marrow stem cells to hepatic lineage cells.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

SAB1306569
ANTI-CYK18(C-TERMINAL) antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
SAB4501665
Anti-Keratin 18, C-Terminal antibody produced in rabbit, affinity isolated antibody
SAB4300551
Anti-KRT18 (Ab-33) antibody produced in rabbit, affinity isolated antibody
AV40205
Anti-KRT18 (AB1) antibody produced in rabbit, IgG fraction of antiserum
AV40206
Anti-KRT18 (AB2) antibody produced in rabbit, IgG fraction of antiserum
GW22753 Anti-KRT18 antibody produced in chicken, affinity isolated antibody, buffered aqueous solution
SAB2701928
Anti-krt18 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
SAB3300015
Monoclonal Anti-CK18 antibody produced in mouse, clone 10, purified immunoglobulin, buffered aqueous glycerol solution
SAB3300017 Monoclonal Anti-CK18 antibody produced in mouse, clone 1H10, purified immunoglobulin, buffered aqueous glycerol solution
SAB1305526
MONOCLONAL ANTI-CYK18 (C-TERMINAL) antibody produced in mouse, clone 695CT17.2.6, crude ascites, buffered aqueous solution
SAB1305562
MONOCLONAL ANTI-CYK18(C-TERMINAL) antibody produced in mouse, clone 695CT17.2.6, IgG fraction of antiserum, buffered aqueous solution
WH0003875M1
Monoclonal Anti-KRT18 antibody produced in mouse, clone 2F8, purified immunoglobulin, buffered aqueous solution
SAB1404012
Monoclonal Anti-KRT18 antibody produced in mouse, clone 1G8, purified immunoglobulin, buffered aqueous solution
SAB4700411
Monoclonal Anti-KRT18 antibody produced in mouse, clone C-04, purified immunoglobulin, buffered aqueous solution
SAB4700670 Monoclonal Anti-KRT18 antibody produced in mouse, clone DC-10, purified immunoglobulin, buffered aqueous solution