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International journal of clinical and experimental medicine

Role of dendritic cell-mediated abnormal immune response in visceral hypersensitivity.


PMID 26550249

Abstract

The role of dendritic cells (DCs) in irritable bowel syndrome (IBS) is unclear. This study tested the hypothesis that intestinal DCs induced visceral hypersensitivity in IBS rats through mast cell (MC) activation. The IBS rat model was established by combining colorectal distension with restraint stress. The number of CD103-positive cells in colon was higher in the IBS group. Expression of PAR-2, IL-4 and IL-9 in the colonic mucosa was higher in the IBS group. Mesenteric lymph node DCs (MLNDCs) and splenic CD4(+)/CD8(+) T cells were isolated and purified by a magnetic labeling-based technique; they were cultured alone or co-cultured (T4+DC/T8+DC). The coculture of MLNDCs and CD4(+) T cells had the highest IL-4 secretion in the IBS group, while IL-9 expression was higher in the cultures containing CD8(+) T cells. Our findings indicate that an increased number of DCs in the colon stimulated CD4(+) T cells to secrete high levels of IL-4, which led to the activation of MCs and subsequently resulted in visceral hypersensitivity.