EMAIL THIS PAGE TO A FRIEND

Scientific reports

Quercitrin for periodontal regeneration: effects on human gingival fibroblasts and mesenchymal stem cells.


PMID 26558438

Abstract

Periodontal disease (PD) is the result of an infection and chronic inflammation of the gingiva that may lead to its destruction and, in severe cases, alveolar bone and tooth loss. The ultimate goal of periodontal treatment is to achieve periodontal soft and hard tissues regeneration. We previously selected quercitrin, a catechol-containing flavonoid, as a potential agent for periodontal applications. In this study, we tested the ability of quercitrin to alter biomarker production involved in periodontal regeneration on primary human gingival fibroblasts (hGF) and primary human mesenchymal stem cells (hMSC) cultured under basal and inflammatory conditions. To mimic PD inflammatory status, interleukin-1 beta (IL-1β) was used. The expression of different genes related to inflammation and extracellular matrix were evaluated and prostaglandin E2 (PGE2) production was quantified in hGFs; alkaline phosphatase (ALP) activity and calcium content were analysed in hMSCs. Quercitrin decreased the release of the inflammatory mediator PGE2 and partially re-established the impaired collagen metabolism induced by IL-1β treatment in hGFs. Quercitrin also increased ALP activity and mineralization in hMSCs, thus, it increased hMSCs differentiation towards the osteoblastic lineage. These findings suggest quercitrin as a novel bioactive molecule with application to enhance both soft and hard tissue regeneration of the periodontium.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

H1758
Hydrochloric acid, 36.5-38.0%, BioReagent, for molecular biology
HCl
435570
Hydrochloric acid, reagent grade, 37%
HCl
40233
Hydrochloric acid, semiconductor grade PURANAL (Honeywell 17863), ≥32%
HCl
40253
Hydrochloric acid, semiconductor grade PURANAL (Honeywell 17823), fuming 37%, 37-38%
HCl
84429
Hydrochloric acid solution, ~6 M in H2O, for amino acid analysis
HCl
H9892
Hydrochloric acid solution, 1.0 N, BioReagent, suitable for cell culture
HCl
84434
Hydrochloric acid solution, BioXtra, ~0.1 M in H2O
HCl
H0636
Hydrochloric acid solution, protein sequencing grade, liquid
HCl
2104
Hydrochloric acid solution, volumetric, 0.1 M HCl (0.1N), endotoxin free
HCl
W530574
Hydrochloric acid solution, 32 wt. % in H2O, FCC
HCl
295426
Hydrogen chloride, ReagentPlus®, ≥99%
HCl
705551
Hydrogen chloride solution, 3 M in cyclopentyl methyl ether (CPME)
HCl
A5960
L-Ascorbic acid, BioXtra, ≥99.0%, crystalline
C6H8O6
A7506
L-Ascorbic acid, reagent grade, crystalline
C6H8O6
A0278
L-Ascorbic acid, reagent grade
C6H8O6
A4403
L-Ascorbic acid, powder, cell culture tested, γ-irradiated
C6H8O6
A4544
L-Ascorbic acid, suitable for cell culture, suitable for plant cell culture, ≥98%
C6H8O6
A2218
L-Ascorbic acid, meets USP testing specifications
C6H8O6
A2174
L-Ascorbic acid, plant cell culture tested
C6H8O6
A2343
L-Ascorbic acid, meets USP testing specifications
C6H8O6
95209
L-Ascorbic acid, BioUltra, ≥99.5% (RT)
C6H8O6
33034
L-Ascorbic acid, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., 99.7-100.5% (oxidimetric)
C6H8O6
95210
L-Ascorbic acid, puriss. p.a., ≥99.0% (RT)
C6H8O6
47863
L-Ascorbic acid, analytical standard
C6H8O6
255564
L-Ascorbic acid, ACS reagent, ≥99%
C6H8O6
A92902
L-Ascorbic acid, 99%
C6H8O6
W210901
L-Ascorbic acid, FCC, FG
C6H8O6
40104-U Methanolic HCl, 3 M HCl in methanol (3N), for GC derivatization, pkg of 1 × 2 L
HCl
P5640
Prostaglandin E2, ≥93% (HPLC), synthetic
C20H32O5
P6532
Prostaglandin E2, γ-irradiated, powder, BioXtra, suitable for cell culture
C20H32O5