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Trends in microbiology

Finally, Archaea Get Their CRISPR-Cas Toolbox.


PMID 28391963

Abstract

The majority of archaea encode CRISPR-Cas systems but only a few CRISPR-Cas-based genetic tools have been developed for organisms from this domain. Nayak and Metcalf have harnessed a bacterial Cas9 protein for genome editing in Methanosarcina acetivorans, enabling efficient gene deletion and replacement.