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Neuron

Evidence for Mechanosensitive Channel Activity of Tentonin 3/TMEM150C.


PMID 28426962

Abstract

Mechanosensation is essential for various physiological processes, and it is mediated by mechanotransduction channels. Recently, we reported that TMEM150C/Tentonin 3 (TTN3) confers mechanically activated currents with slow inactivation kinetics in several cell types, including dorsal root ganglion neurons (Hong etxa0al., 2016). The accompanying Matters Arising by Dubin, Murthy, and colleagues confirms that naive heterologous cells demonstrate a mechanically activated current, but finds that this response is absent in CRISPR-Cas9 Piezo1 knockout cell lines and suggests that TTN3 is a modulator of Piezo1. We present and discuss evidence based on co-expression of TTN3 and Peizo1 and mutant variants of the pore region of TTN3 to support that TTN3 isxa0axa0pore-forming unit, not an amplifying adaptor forxa0Piezo1 activity. This Matters Arising Response paper, along with Zhao etxa0al. (2017), addresses the Matters Arising from Dubin etxa0al. (2017), published concurrently in this issue of Neuron.